Genome annotation for eukaryotes

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We recommend you follow the tutorials in the order presented on this page. They have been selected to fit together and build up your knowledge step by step. If a lesson has both slides and a tutorial, we recommend you start with the slides, then proceed with the tutorial.

Learn how to annotate an eukaryotic genome sequence: identify repeated regions, find the position and function of genes, and even set up a manual curation environment with Apollo.

Module 1: Introduction

General introduction to genome annotation

Lesson Slides Hands-on Recordings
Introduction to Genome Annotation

Module 2: Repeat Masking

Learn how to identifying, and “mask”, repeated regions, a first step before annotating genes

Lesson Slides Hands-on Recordings
Masking repeats with RepeatMasker

Module 3: Gene calling and functional annotation

In this module you will learn to run the Funannotate tool suite to find the position of genes and to functionally annotate them. Optionally you can also identify long non-coding RNAs.

Lesson Slides Hands-on Recordings
Genome annotation with Funannotate
Long non-coding RNAs (lncRNAs) annotation with FEELnc

Module 4: Manual curation

Automatic annotation is rarely perfect, in this module you will learn how to start a collaborative manual curation project using Galaxy and Apollo

Lesson Slides Hands-on Recordings
Refining Genome Annotations with Apollo (eukaryotes)

Editorial Board

This material is reviewed by our Editorial Board:

orcid logoAnthony Bretaudeau avatar Anthony Bretaudeau


This material was funded by:

Gallantries: Bridging Training Communities in Life Science, Environment and Health avatar GallantriesEuroScienceGateway avatar EuroScienceGateway