MGnify's amplicon pipeline v5.0 - Quality control PE
microbiome-mgnify-amplicon/mgnify-amplicon-pipeline-v5-quality-control-paired-end
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flowchart TD 0["ℹ️ Input Collection\nPaired-end reads"]; style 0 stroke:#2c3143,stroke-width:4px; 1["ℹ️ Input Parameter\nfastp - Enable base correction"]; style 1 fill:#ded,stroke:#393,stroke-width:4px; 2["ℹ️ Input Parameter\nfastp - Qualified quality phred"]; style 2 fill:#ded,stroke:#393,stroke-width:4px; 3["ℹ️ Input Parameter\nfastp - Unqualified percent limit "]; style 3 fill:#ded,stroke:#393,stroke-width:4px; 4["ℹ️ Input Parameter\nfastp - Length required"]; style 4 fill:#ded,stroke:#393,stroke-width:4px; 5["ℹ️ Input Parameter\nTrimmomatic - SLIDINGWINDOW - Number of bases to average across"]; style 5 fill:#ded,stroke:#393,stroke-width:4px; 6["ℹ️ Input Parameter\nTrimmomatic - SLIDINGWINDOW - Average quality required"]; style 6 fill:#ded,stroke:#393,stroke-width:4px; 7["ℹ️ Input Parameter\nTrimmomatic - LEADING"]; style 7 fill:#ded,stroke:#393,stroke-width:4px; 8["ℹ️ Input Parameter\nTrimmomatic - TRAILING"]; style 8 fill:#ded,stroke:#393,stroke-width:4px; 9["ℹ️ Input Parameter\nLength filtering - Minimum size"]; style 9 fill:#ded,stroke:#393,stroke-width:4px; 10["ℹ️ Input Parameter\nTrimmomatic - MINLEN"]; style 10 fill:#ded,stroke:#393,stroke-width:4px; 11["ℹ️ Input Parameter\nAmbiguity filtering - Maximal N percentage threshold to conserve sequences"]; style 11 fill:#ded,stroke:#393,stroke-width:4px; 12["fastp"]; 4 -->|output| 12; 2 -->|output| 12; 3 -->|output| 12; 1 -->|output| 12; 0 -->|output| 12; 13["Unzip collection"]; 12 -->|output_paired_coll| 13; 14["Merging paired-end Illumina reads SeqPrep, modified for use with MGnify piplines"]; 13 -->|forward| 14; 13 -->|reverse| 14; 15["Trimming"]; 6 -->|output| 15; 5 -->|output| 15; 7 -->|output| 15; 8 -->|output| 15; 10 -->|output| 15; 14 -->|merged| 15; 16["FastQC"]; 14 -->|merged| 16; 17["Length filtering"]; 15 -->|fastq_out| 17; 9 -->|output| 17; 18["FastQC"]; 15 -->|fastq_out| 18; 19["Replace"]; 16 -->|text_file| 19; 20["Ambiguity filtering"]; 11 -->|output| 20; 17 -->|output_file| 20; 21["FastQC"]; 17 -->|output_file| 21; 22["Replace"]; 18 -->|text_file| 22; 23["FASTQ to FASTA"]; 20 -->|good_sequence_file| 23; 24["FastQC"]; 20 -->|good_sequence_file| 24; 25["Replace"]; 21 -->|text_file| 25; 26["FASTA Width"]; 23 -->|output_file| 26; 05008f5e-c45d-4346-8e85-ec2fb5877a95["Output\nPaired-end post quality control FASTA files"]; 26 --> 05008f5e-c45d-4346-8e85-ec2fb5877a95; style 05008f5e-c45d-4346-8e85-ec2fb5877a95 stroke:#2c3143,stroke-width:4px; 27["Replace"]; 24 -->|text_file| 27; 28["MultiQC"]; 19 -->|outfile| 28; 22 -->|outfile| 28; 25 -->|outfile| 28; 27 -->|outfile| 28; 82fc1b1a-80b4-4abf-8150-cca1d82337bb["Output\nPaired-end MultiQC report"]; 28 --> 82fc1b1a-80b4-4abf-8150-cca1d82337bb; style 82fc1b1a-80b4-4abf-8150-cca1d82337bb stroke:#2c3143,stroke-width:4px; 6b214cd1-fd0b-4d57-89f4-5758e4ad0c32["Output\nPaired-end MultiQC statistics"]; 28 --> 6b214cd1-fd0b-4d57-89f4-5758e4ad0c32; style 6b214cd1-fd0b-4d57-89f4-5758e4ad0c32 stroke:#2c3143,stroke-width:4px;
Inputs
Input | Label |
---|---|
Input dataset collection | Paired-end reads |
Input parameter | fastp - Enable base correction |
Input parameter | fastp - Qualified quality phred |
Input parameter | fastp - Unqualified percent limit |
Input parameter | fastp - Length required |
Input parameter | Trimmomatic - SLIDINGWINDOW - Number of bases to average across |
Input parameter | Trimmomatic - SLIDINGWINDOW - Average quality required |
Input parameter | Trimmomatic - LEADING |
Input parameter | Trimmomatic - TRAILING |
Input parameter | Length filtering - Minimum size |
Input parameter | Trimmomatic - MINLEN |
Input parameter | Ambiguity filtering - Maximal N percentage threshold to conserve sequences |
Outputs
From | Output | Label |
---|---|---|
toolshed.g2.bx.psu.edu/repos/devteam/fasta_formatter/cshl_fasta_formatter/1.0.1+galaxy2 | FASTA Width | |
toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.27+galaxy3 | MultiQC |
Tools
To use these workflows in Galaxy you can either click the links to download the workflows, or you can right-click and copy the link to the workflow which can be used in the Galaxy form to import workflows.
Importing into Galaxy
Below are the instructions for importing these workflows directly into your Galaxy server of choice to start using them!Hands On: Importing a workflow
- Click on galaxy-workflows-activity Workflows in the Galaxy activity bar (on the left side of the screen, or in the top menu bar of older Galaxy instances). You will see a list of all your workflows
- Click on galaxy-upload Import at the top-right of the screen
- Provide your workflow
- Option 1: Paste the URL of the workflow into the box labelled “Archived Workflow URL”
- Option 2: Upload the workflow file in the box labelled “Archived Workflow File”
- Click the Import workflow button
Below is a short video demonstrating how to import a workflow from GitHub using this procedure:
Video: Importing a workflow from URL
Version History
Version | Commit | Time | Comments |
---|---|---|---|
1 | 0125a7f84 | 2025-07-30 12:59:47 | remove version from folder name for future-proofness |
For Admins
Installing the workflow tools
wget https://training.galaxyproject.org/training-material/topics/microbiome/tutorials/mgnify-amplicon/workflows/mgnify-amplicon-pipeline-v5-quality-control-paired-end.ga -O workflow.ga workflow-to-tools -w workflow.ga -o tools.yaml shed-tools install -g GALAXY -a API_KEY -t tools.yaml workflow-install -g GALAXY -a API_KEY -w workflow.ga --publish-workflows