New Galaxy training: Building an amplicon sequence variant (ASV) table from 16S data using DADA2

Author(s) orcid logoBérénice Batut avatar Bérénice Batut
new tutorial microbiome

Posted on: 6 June 2024 PURL: https://gxy.io/GTN:N00085

The GTN hosts its 400th tutorial: Building an amplicon sequence variant (ASV) table from 16S data using DADA2

The investigation of environmental microbial communities and microbiomes has been revolutionized by the development of high-throughput amplicon sequencing. In amplicon sequencing a particular genetic locus, for example the 16S rRNA gene (or a part of it) in bacteria, is amplified from DNA extracted from the community of interest, and then sequenced on a next-generation sequencing platform. This technique removes the need to culture microbes in order to detect their presence, and cost-effectively provides a deep census of a microbial community.

This new Galaxy training explores 16S amplicon data processing using DADA2 and exploration using Phyloseq.

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