In this tutorial we are going to explore the basics of using Jupyter in Galaxy. We will use a RNA seq count file as a test set to get a hang of the Jupyter notebooks. The file is available in Zenodo or in the Tutorial section of Data Libraries. Select a file ending with .counts and upload it in your history (If you want to know how to upload data in galaxy, see Getting Data into Galaxy tutorial)

Agenda

In this tutorial, we will see :

1. What is Jupyter ?
2. Use Jupyter notebook in Galaxy
   1. Open a Notebook
   2. Install Libraries in Jupyter
   3. Graph Display in Jupyter
   4. Import / export Data
   5. Save the Notebook in your history
3. Conclusion

What is Jupyter ?

Jupyter in an interactive environment that mixes explanatory text, command line and output display for an interactive analysis environment. Its implementation in Galaxy facilitate the performance of additional analysis if there is no tool for it.

These notebooks allow you to replace any in-house script you might need to complete your analysis. You don’t need to move your data out of Galaxy. You can describe each step of your analysis in the markdown cells for an easy understanding of the processes, and save it in your history for sharing and reproducibility. In addition, thanks to Jupyter magic commands, you can use several different languages in a single notebook.

You can find the complete manual for Jupyter commands on Read the Docs.

Use Jupyter notebook in Galaxy

Open a Notebook

The Jupyter notebook can be started from different points. You can either open a Jupyter notebook from a dataset in your history or from the Visualize tab in the upper menu.

Hands On: Launching a Jupyter notebook from a dataset or a saved Jupyter notebook

If you only need one dataset from your history to perform you analysis or want to open a Jupyter notebook that you previously saved in your history, you can launch a Jupyter from a single dataset.

1. Expand the dataset in you history by clicking on its name.
2. Click on the visualization icon galaxy-barchart of the dataset [...].counts.
3. Select the Jupyter visualization in the list.

Hands On: Lauching a Jupyter notebook from the Visualize tab

1. Click on the Visualize tab on the upper menu and select Interactive Environments
2. To open a notebook, set the parameters as follows :
   • “GIE” : Jupyter
   • “Image” : quay.io/bgruening/docker-jupyter-notebook:17.09
   • “Datasets” : The datasets you want to work on, here your [...].counts file. If the first dataset you select is a notebook from you history, it will be opened instead of a new notebook.
3. Click Launch

Install Libraries in Jupyter

You can install tools and libraries in Jupyter through conda and pip. In this tutorial we are going to use two libraries, pandas and seaborn respectively allowing to manipulate data as Dataframe and to create graphs.

Hands On: Install from a Conda recipe

1. Click on a cell of your notebook to edit it (verify that it is defined as a Code cell)
2. Enter the following lines : !conda install -y pandas and !conda install -y seaborn
   • The ! indicate you are typing a bash command line (alternatively you can use %%bash at the beginning of your cell )
   • The -y option allows the installation without asking for confirmation (The confirmation is not managed well by notebooks)
3. shift+return to run the cell or click on the run cell button.

Hands On: Import Python libraries

1. Click on a cell of your notebook to edit it (verify that it is defined as a Code cell)
2. Enter the following lines : import pandas as pd, import seaborn as sns, from IPython.display import display, and import matplotlib.pyplot as plt.
3. shift+return to run the cell or click on the run cell button.

Graph Display in Jupyter

In this tutorial we are going to simply plot a distribution graph of our data.

Hands On: Draw a distribution plot

1. Open the dataset as a pandas Dataframe with the function dataframe = pd.read_table("[file_number]", header=None)
   • The files are referenced in Jupyter by their number in the history.
2. Create your figure with the command fig, ax = plt.subplots( nrows=1, ncols=1 ,figsize=(15, 10) )
   • nrows=1, ncols=1 means you will have one plot in your figure (one row and one column)
   • figsize parameter determine the size of the figure
3. Draw the distribution plot of the second column of our dataset with the command sns.distplot(dataframe[1]);
4. Show the figure in the Jupyter notebook with display(fig)

Import / export Data

In addition of starting a Jupyter notebook with datasets included at the beginning , you can import them later using the get(12) command, with the number of your dataset in the history (If you are working on a collection, unhide datasets to see their numbers). If you want to save a file you generated in your notebook, use the put("file_name") command. That is what we are going to do with our distribution plot.

Hands On: Save an Jupyter generated image into a Galaxy History

1. Create an image file with the figure you just draw with the command fig.savefig('distplot.png')
2. Export your image into your history with the command put('distplot.png')

Save the Notebook in your history

Once you are done with you analysis or anytime during the editing process, you can save the notebook into your history by clicking on the Save icon.

This will create a new notebook .pynb file in your history every time you click on this icon. You can later re-open it to continue to use it as described in the open a notebook section

Conclusion

trophy You have just performed your first analysis in Jupyter notebook integrated environment in Galaxy. You generated an distribution plot that you saved in your history along with the notebook to generate it.

You've Finished the Tutorial

Please also consider filling out the Feedback Form as well!

Key points

• Start Jupyter from the Visualize tab or from a dataset

• Install Libraries with pip or Conda

• Use get() to import datasets from your history to the notebook

• Use put() to export datasets from the notebook to your history

• Save your notebook into your history

Frequently Asked Questions

• Tutorial FAQs
• Topic FAQs
• Galaxy FAQs
• GTN Matrix Chat
• Galaxy Help Forum

Feedback

Did you use this material as an instructor? Feel free to give us feedback on how it went.
Did you use this material as a learner or student? Click the form below to leave feedback.

Citing this Tutorial

1. Delphine Lariviere, Use Jupyter notebooks in Galaxy (Galaxy Training Materials). https://training.galaxyproject.org/training-material/topics/galaxy-interface/tutorials/galaxy-intro-jupyter/tutorial.html Online; accessed TODAY
2. Hiltemann, Saskia, Rasche, Helena et al., 2023 Galaxy Training: A Powerful Framework for Teaching! PLOS Computational Biology 10.1371/journal.pcbi.1010752
3. Batut et al., 2018 Community-Driven Data Analysis Training for Biology Cell Systems 10.1016/j.cels.2018.05.012

BibTeX

@misc{galaxy-interface-galaxy-intro-jupyter,
author = "Delphine Lariviere",
	title = "Use Jupyter notebooks in Galaxy (Galaxy Training Materials)",
	year = "",
	month = "",
	day = "",
	url = "\url{https://training.galaxyproject.org/training-material/topics/galaxy-interface/tutorials/galaxy-intro-jupyter/tutorial.html}",
	note = "[Online; accessed TODAY]"
}
@article{Hiltemann_2023,
	doi = {10.1371/journal.pcbi.1010752},
	url = {https://doi.org/10.1371%2Fjournal.pcbi.1010752},
	year = 2023,
	month = {jan},
	publisher = {Public Library of Science ({PLoS})},
	volume = {19},
	number = {1},
	pages = {e1010752},
	author = {Saskia Hiltemann and Helena Rasche and Simon Gladman and Hans-Rudolf Hotz and Delphine Larivi{\`{e}}re and Daniel Blankenberg and Pratik D. Jagtap and Thomas Wollmann and Anthony Bretaudeau and Nadia Gou{\'{e}} and Timothy J. Griffin and Coline Royaux and Yvan Le Bras and Subina Mehta and Anna Syme and Frederik Coppens and Bert Droesbeke and Nicola Soranzo and Wendi Bacon and Fotis Psomopoulos and Crist{\'{o}}bal Gallardo-Alba and John Davis and Melanie Christine Föll and Matthias Fahrner and Maria A. Doyle and Beatriz Serrano-Solano and Anne Claire Fouilloux and Peter van Heusden and Wolfgang Maier and Dave Clements and Florian Heyl and Björn Grüning and B{\'{e}}r{\'{e}}nice Batut and},
	editor = {Francis Ouellette},
	title = {Galaxy Training: A powerful framework for teaching!},
	journal = {PLoS Comput Biol}
}

                   

Galaxy Administrators: Install the missing tools

You can use Ephemeris's shed-tools install command to install the tools used in this tutorial.

shed-tools install [-g GALAXY] [-a API_KEY] -t <(curl https://training.galaxyproject.org/training-material/api/topics/galaxy-interface/tutorials/galaxy-intro-jupyter/tutorial.json | jq .admin_install_yaml -r)

Alternatively you can copy and paste the following YAML

---
install_tool_dependencies: true
install_repository_dependencies: true
install_resolver_dependencies: true
tools: []

Feedback

t{ hist[0] | to_stars }}	1
t{ hist[0] | to_stars }}	2
t{ hist[0] | to_stars }}	1

January 2023

• 3 stars: Liked: It got me started on how to use the interactive jupyter tool. However, it issuing python 2 instead of python 3 which is currently used by use galaxy.org . Disliked: 1. Should use python 3 instead of python 2. 2. distplot in version of seborn in usegalaxy.org is deprecated.. 3. I cannot find the download button to save notebook to history file. Instead I downloaded it to my composer and can subsequently upload it to use galaxy.org. 4. It would be easier if the code on the webpage was set apart from the text as a code block with the ability to copy it by clicking on copy icon in the code block.

November 2022

• 2 stars: Disliked: The instructions may not be up to date. I tried following them but what was described did not appear when I tried to follow. For example, there is no jupyter icon that comes up under visualizations.

December 2021

• 2 stars: Liked: was well explained. Disliked: The tutorial looks nothing like what is one my galaxy web instance. Jupiter notebooks are not listed under visualizations but rather are under tools. The dialog that comes up when I launch Jupyter also does not look like what is in this tutorial. I can't figure it out based on what you have shown.