Imaging

Image analysis using Galaxy

Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides () and tutorial () buttons below.

Introduction

Start here if you are new to Image Analysis in Galaxy

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Image Analysis using Galaxy
Image segmentation Image thresholding Conversion Object counting Overlay Fluorescence microscopy
Overview of the Galaxy OMERO-suite - Upload images and metadata in OMERO using Galaxy
Data management Data handling Data sharing ROI selection
Where to start with bioimage analysis in Galaxy
Microscopy Bioimaging Histology
FAIR Bioimage Metadata
Data management Metadata Bioimaging
REMBI - Recommended Metadata for Biological Images – metadata guidelines for bioimaging data
Data management Metadata Bioimaging

Analyses

These tutorials will help you analyse your image data

Lesson Slides Hands-on Recordings Input dataset Workflows
Analyse HeLa fluorescence siRNA screen
Image segmentation Object feature extraction Image thresholding High-throughput screening Overlay Fluorescence microscopy
End-to-End Tissue Microarray Image Analysis with Galaxy-ME
Image segmentation Tissue imaging
Nucleoli segmentation and feature extraction using CellProfiler
Image segmentation Object feature extraction Multi-channel image
Object tracking using CellProfiler
Isolated object tracking Multi-channel image
Quantification of electrophoresis gel bands using QuPath and Galaxy imaging tools
ROI selection Conversion Object feature extraction Multi-channel image
Quantification of single-molecule RNA fluorescence in situ hybridization (smFISH) in yeast cell lines
IDR dataset Spot detection Rasterisation Overlay
Segmentation of Anatomical Structures in Medical 3-D Images
Image segmentation Conversion Overlay Object feature extraction Medical imaging Computed tomography 3D image Volume rendering
Tracking of mitochondria and capturing mitoflashes
Isolated object tracking Temporal analysis Time-lapse data
Using BioImage.IO models for image analysis in Galaxy
Image segmentation Image annotation Deep learning
Voronoi segmentation
Tessellation Image segmentation Object counting Overlay Multi-channel image

Advanced topics

Tutorials covering advanced topics

Lesson Slides Hands-on Recordings Input dataset Workflows
Content Tracking and Verification in Galaxy Workflows with ISCC-SUM
Data management Data handling Data sharing Validation
Execute a BiaPy workflow in Galaxy
Image segmentation Image annotation Deep learning Conversion Overlay 3D image Volume rendering
Parameter tuning and optimization - Evaluating nuclei segmentation with Galaxy
Image segmentation Parameter optimisation Validation
Training Custom YOLO Models for Segmentation of Bioimages
Image segmentation Image annotation Deep learning

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoLeonid Kostrykin avatar Leonid Kostrykinorcid logoBeatriz Serrano-Solano avatar Beatriz Serrano-Solano

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Contributors

This material was contributed to by:

orcid logoJulia Jakiela avatar Julia Jakielaorcid logoAllison Creason avatar Allison Creasonorcid logoMartin Čech avatar Martin Čechorcid logoPaul De Geest avatar Paul De Geestorcid logoBjörn Grüning avatar Björn Grüningorcid logoPavankumar Videm avatar Pavankumar Videmorcid logoAnne Fouilloux avatar Anne FouillouxYi Sun avatar Yi SunLaura Cooper avatar Laura Cooperorcid logoBeatriz Serrano-Solano avatar Beatriz Serrano-Solanoorcid logoAnup Kumar avatar Anup KumarMaria Arrate Munoz Barrutia avatar Maria Arrate Munoz Barrutiaorcid logoMartin Etzrodt avatar Martin EtzrodtJean-Karim Hériché avatar Jean-Karim HérichéArif ul Maula Khan avatar Arif ul Maula Khanorcid logoDiana Chiang Jurado avatar Diana Chiang Juradoorcid logoArmin Dadras avatar Armin Dadrasorcid logoCameron Watson avatar Cameron Watsonorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoMaarten Paul avatar Maarten Paulorcid logoEven Moa Myklebust avatar Even Moa Myklebustorcid logoKatarzyna Kamieniecka avatar Katarzyna Kamienieckaorcid logoThomas Wollmann avatar Thomas Wollmannorcid logoKrzysztof Poterlowicz avatar Krzysztof PoterlowiczReyhaneh Tavakoli avatar Reyhaneh Tavakoliorcid logoLeonid Kostrykin avatar Leonid KostrykinDaniel Franco Barranco avatar Daniel Franco Barrancoorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoRiccardo Massei avatar Riccardo Masseiorcid logoHelena Rasche avatar Helena Rascheorcid logoKhaled Jum'ah avatar Khaled Jum'ah

Funding

These individuals or organisations provided funding support for the development of this resource

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ELIXIR Europe
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DASH UK
This Fellowship was funded through the ELIXIR-UK DaSH project as part of the UKRI Innovation Scholars: Data Science Training in Health and Bioscience call (DaSH). (MR/V038966/1). The project aims to embed Research Data Management (RDM) know-how into UK universities and institutes by producing and delivering training in FAIR data stewardship using ELIXIR-UK knowledge and resources.
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de.NBI
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DFG
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OSCARS-FIESTA
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NFDI4Bioimage
NFDI4BIOIMAGE is a nationwide community of researchers, imaging specialists and data experts who work with biological image data. We support anyone who generates, analyses or manages microscopy and bioimaging data. Our aim is to make imaging data easier to find, access, reuse and share by providing practical tools, clear guidance and hands on training. We collaborate closely with laboratories and research communities across Germany to understand their needs and develop solutions that help with real everyday challenges. Whether you are building new workflows, exploring advanced analysis methods or improving your data management, NFDI4BIOIMAGE offers support, expertise and a welcoming network.
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UFR
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OSCARS
The OSCARS project has received funding from the European Commission’s Horizon Europe Research and Innovation programme under grant agreement No. 101129751

References