Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoSimon Gladman avatar Simon Gladmanorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

Contributors

This material was contributed to by:

Kenji Fujihara avatar Kenji FujiharaTorsten Seemann avatar Torsten Seemannorcid logoPaul Zierep avatar Paul Ziereporcid logoSimon Gladman avatar Simon GladmanJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoAnna Syme avatar Anna Symeorcid logoBérénice Batut avatar Bérénice BatutTwishi Gulati avatar Twishi GulatiTeresa Müller avatar Teresa MüllerSimon Bray avatar Simon Brayorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoErwan Corre avatar Erwan CorreEkaterina Polkh avatar Ekaterina Polkhorcid logoDelphine Lariviere avatar Delphine LariviereNathan Dunn avatar Nathan DunnWilliam Durand avatar William DurandBazante Sanders avatar Bazante Sandersorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoCristóbal Gallardo avatar Cristóbal GallardoNiall Beard avatar Niall Beardorcid logoAlexandre Cormier avatar Alexandre CormierGildas Le Corguillé avatar Gildas Le CorguilléEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoHelena Rasche avatar Helena Rascheorcid logoMateo Boudet avatar Mateo Boudetorcid logoLaura Leroi avatar Laura LeroiAnton Nekrutenko avatar Anton NekrutenkoDeepti Varshney avatar Deepti Varshneyorcid logoBjörn Grüning avatar Björn Grüningorcid logoMaria Doyle avatar Maria Doyle

Funding

These individuals or organisations provided funding support for the development of this resource