Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

orcid logoStéphanie Robin avatar Stéphanie RobinSimon Bray avatar Simon Brayorcid logoHelena Rasche avatar Helena RascheTeresa Müller avatar Teresa Müllerorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoMateo Boudet avatar Mateo Boudetorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoSaskia Hiltemann avatar Saskia HiltemannWilliam Durand avatar William DurandTorsten Seemann avatar Torsten Seemannorcid logoSimon Gladman avatar Simon Gladmanorcid logoBjörn Grüning avatar Björn GrüningAnton Nekrutenko avatar Anton Nekrutenkoorcid logoAnika Erxleben avatar Anika ErxlebenBazante Sanders avatar Bazante Sandersorcid logoLaura Leroi avatar Laura LeroiEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoErwan Corre avatar Erwan Correorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoMaria Doyle avatar Maria DoyleDeepti Varshney avatar Deepti Varshneyorcid logoMiaomiao Zhou avatar Miaomiao ZhouNiall Beard avatar Niall BeardNathan Dunn avatar Nathan Dunnorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoAnthony Bretaudeau avatar Anthony BretaudeauJasper Ouwerkerk avatar Jasper OuwerkerkEkaterina Polkh avatar Ekaterina Polkhorcid logoPaul Zierep avatar Paul ZierepTwishi Gulati avatar Twishi GulatiKenji Fujihara avatar Kenji Fujiharaorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoBérénice Batut avatar Bérénice BatutGildas Le Corguillé avatar Gildas Le Corguilléorcid logoAnna Syme avatar Anna Syme

Funding

These individuals or organisations provided funding support for the development of this resource