Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoRomane LIBOUBAN avatar Romane LIBOUBANHélène Chiapello avatar Hélène Chiapelloorcid logoTeresa Müller avatar Teresa Müllerorcid logoDeepti Varshney avatar Deepti VarshneyValentin Loux avatar Valentin LouxAnton Nekrutenko avatar Anton Nekrutenkoorcid logoSaim Momin avatar Saim MominEkaterina Polkh avatar Ekaterina Polkhorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoMaria Doyle avatar Maria Doyleorcid logoTristan Reynolds avatar Tristan ReynoldsNathan Dunn avatar Nathan DunnBazante Sanders avatar Bazante Sandersorcid logoAnthony Bretaudeau avatar Anthony BretaudeauSimon Bray avatar Simon Brayorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoHelena Rasche avatar Helena RascheTwishi Gulati avatar Twishi GulatiNiall Beard avatar Niall Beardorcid logoAnika Erxleben avatar Anika ErxlebenEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoLaura Leroi avatar Laura Leroiorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoErwan Corre avatar Erwan CorreTorsten Seemann avatar Torsten Seemannorcid logoNiklas Mayle avatar Niklas MayleGildas Le Corguillé avatar Gildas Le Corguilléorcid logoPaul Zierep avatar Paul ZierepJasper Ouwerkerk avatar Jasper OuwerkerkWilliam Durand avatar William DurandGuillaume Gautreau avatar Guillaume GautreauKenji Fujihara avatar Kenji Fujiharaorcid logoBjörn Grüning avatar Björn Grüningorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoAnna Syme avatar Anna Symeorcid logoSimon Gladman avatar Simon Gladmanorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoMateo Boudet avatar Mateo Boudetorcid logoBérénice Batut avatar Bérénice Batutorcid logoFelicitas Kindel avatar Felicitas Kindel

Funding

These individuals or organisations provided funding support for the development of this resource