Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoNiklas Mayle avatar Niklas Mayleorcid logoBérénice Batut avatar Bérénice Batutorcid logoDeepti Varshney avatar Deepti VarshneyBazante Sanders avatar Bazante SandersWilliam Durand avatar William Durandorcid logoMiaomiao Zhou avatar Miaomiao ZhouKenji Fujihara avatar Kenji FujiharaSimon Bray avatar Simon BrayNiall Beard avatar Niall Beardorcid logoTeresa Müller avatar Teresa MüllerGuillaume Gautreau avatar Guillaume Gautreauorcid logoMaria Doyle avatar Maria Doyleorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoErwan Corre avatar Erwan Correorcid logoHelena Rasche avatar Helena Rascheorcid logoDelphine Lariviere avatar Delphine LariviereNathan Dunn avatar Nathan Dunnorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoBjörn Grüning avatar Björn GrüningEkaterina Polkh avatar Ekaterina Polkhorcid logoSimon Gladman avatar Simon Gladmanorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoMateo Boudet avatar Mateo Boudetorcid logoSaim Momin avatar Saim Mominorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoPaul Zierep avatar Paul Ziereporcid logoNicola Soranzo avatar Nicola SoranzoTorsten Seemann avatar Torsten SeemannHélène Chiapello avatar Hélène ChiapelloGildas Le Corguillé avatar Gildas Le CorguilléTwishi Gulati avatar Twishi GulatiJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoLaura Leroi avatar Laura Leroiorcid logoSaskia Hiltemann avatar Saskia HiltemannValentin Loux avatar Valentin Louxorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoAnna Syme avatar Anna SymeEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoCristóbal Gallardo avatar Cristóbal GallardoAnton Nekrutenko avatar Anton Nekrutenko

Funding

These individuals or organisations provided funding support for the development of this resource