Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoBérénice Batut avatar Bérénice Batutorcid logoNiklas Mayle avatar Niklas MayleGuillaume Gautreau avatar Guillaume Gautreauorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoAlexandre Cormier avatar Alexandre CormierEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilKenji Fujihara avatar Kenji FujiharaGildas Le Corguillé avatar Gildas Le CorguilléNiall Beard avatar Niall Beardorcid logoSimon Gladman avatar Simon Gladmanorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoBjörn Grüning avatar Björn GrüningNathan Dunn avatar Nathan DunnSimon Bray avatar Simon Brayorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoPaul Zierep avatar Paul ZierepWilliam Durand avatar William DurandTwishi Gulati avatar Twishi Gulatiorcid logoLaura Leroi avatar Laura Leroiorcid logoStéphanie Robin avatar Stéphanie RobinValentin Loux avatar Valentin Louxorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoJonathan Kreplak avatar Jonathan KreplakBazante Sanders avatar Bazante Sandersorcid logoMateo Boudet avatar Mateo Boudetorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoAnna Syme avatar Anna Symeorcid logoAnika Erxleben avatar Anika ErxlebenHélène Chiapello avatar Hélène Chiapelloorcid logoDeepti Varshney avatar Deepti VarshneyTorsten Seemann avatar Torsten Seemannorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoTeresa Müller avatar Teresa MüllerAnton Nekrutenko avatar Anton NekrutenkoJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoErwan Corre avatar Erwan Correorcid logoMaria Doyle avatar Maria Doyleorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoSaim Momin avatar Saim Mominorcid logoHelena Rasche avatar Helena RascheEkaterina Polkh avatar Ekaterina Polkh

Funding

These individuals or organisations provided funding support for the development of this resource