Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Valentin Loux avatar Valentin LouxBazante Sanders avatar Bazante Sandersorcid logoErwan Corre avatar Erwan Correorcid logoTeresa Müller avatar Teresa Müllerorcid logoLaura Leroi avatar Laura Leroiorcid logoMateo Boudet avatar Mateo Boudetorcid logoSimon Gladman avatar Simon Gladmanorcid logoSaim Momin avatar Saim Mominorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoAnna Syme avatar Anna SymeEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoDelphine Lariviere avatar Delphine LariviereTwishi Gulati avatar Twishi Gulatiorcid logoMiaomiao Zhou avatar Miaomiao ZhouNathan Dunn avatar Nathan Dunnorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoMaria Doyle avatar Maria DoyleJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoBérénice Batut avatar Bérénice Batutorcid logoNiklas Mayle avatar Niklas MayleKenji Fujihara avatar Kenji Fujiharaorcid logoJonathan Kreplak avatar Jonathan KreplakAnton Nekrutenko avatar Anton Nekrutenkoorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoCristóbal Gallardo avatar Cristóbal GallardoTorsten Seemann avatar Torsten Seemannorcid logoRomane LIBOUBAN avatar Romane LIBOUBANEkaterina Polkh avatar Ekaterina PolkhGildas Le Corguillé avatar Gildas Le Corguilléorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoTristan Reynolds avatar Tristan ReynoldsHélène Chiapello avatar Hélène Chiapelloorcid logoBjörn Grüning avatar Björn Grüningorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoPaul Zierep avatar Paul ZierepWilliam Durand avatar William Durandorcid logoHelena Rasche avatar Helena Rascheorcid logoAnika Erxleben avatar Anika ErxlebenGuillaume Gautreau avatar Guillaume GautreauNiall Beard avatar Niall BeardSimon Bray avatar Simon Bray

Funding

These individuals or organisations provided funding support for the development of this resource