Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Hélène Chiapello avatar Hélène Chiapelloorcid logoDelphine Lariviere avatar Delphine LariviereTorsten Seemann avatar Torsten SeemannValentin Loux avatar Valentin LouxBazante Sanders avatar Bazante Sandersorcid logoBérénice Batut avatar Bérénice Batutorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoErwan Corre avatar Erwan Correorcid logoDeepti Varshney avatar Deepti VarshneyGuillaume Gautreau avatar Guillaume Gautreauorcid logoCristóbal Gallardo avatar Cristóbal GallardoWilliam Durand avatar William Durandorcid logoNicola Soranzo avatar Nicola SoranzoGildas Le Corguillé avatar Gildas Le Corguilléorcid logoAnthony Bretaudeau avatar Anthony BretaudeauEkaterina Polkh avatar Ekaterina PolkhAnton Nekrutenko avatar Anton NekrutenkoEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoPaul Zierep avatar Paul Ziereporcid logoMateo Boudet avatar Mateo Boudetorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoAnika Erxleben avatar Anika ErxlebenNathan Dunn avatar Nathan Dunnorcid logoAnna Syme avatar Anna SymeKenji Fujihara avatar Kenji Fujiharaorcid logoFelicitas Kindel avatar Felicitas KindelTwishi Gulati avatar Twishi Gulatiorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoBjörn Grüning avatar Björn GrüningNiall Beard avatar Niall Beardorcid logoHelena Rasche avatar Helena Rascheorcid logoRomane LIBOUBAN avatar Romane LIBOUBANSimon Bray avatar Simon Brayorcid logoTeresa Müller avatar Teresa Müllerorcid logoSimon Gladman avatar Simon Gladmanorcid logoMaria Doyle avatar Maria DoyleJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoLaura Leroi avatar Laura Leroiorcid logoAlexandre Cormier avatar Alexandre Cormier

Funding

These individuals or organisations provided funding support for the development of this resource