Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Torsten Seemann avatar Torsten SeemannSimon Bray avatar Simon Brayorcid logoPaul Zierep avatar Paul ZierepJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoMaria Doyle avatar Maria Doyleorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoRomane LIBOUBAN avatar Romane LIBOUBANGuillaume Gautreau avatar Guillaume GautreauHélène Chiapello avatar Hélène ChiapelloGildas Le Corguillé avatar Gildas Le Corguilléorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoLaura Leroi avatar Laura Leroiorcid logoBjörn Grüning avatar Björn GrüningNiall Beard avatar Niall Beardorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoTeresa Müller avatar Teresa Müllerorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoHelena Rasche avatar Helena Rascheorcid logoAlexandre Cormier avatar Alexandre CormierValentin Loux avatar Valentin Louxorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoAnna Syme avatar Anna Symeorcid logoSimon Gladman avatar Simon Gladmanorcid logoMateo Boudet avatar Mateo Boudetorcid logoErwan Corre avatar Erwan Correorcid logoBérénice Batut avatar Bérénice Batutorcid logoAnthony Bretaudeau avatar Anthony BretaudeauTwishi Gulati avatar Twishi GulatiEkaterina Polkh avatar Ekaterina PolkhNathan Dunn avatar Nathan Dunnorcid logoAnika Erxleben avatar Anika ErxlebenWilliam Durand avatar William DurandAnton Nekrutenko avatar Anton NekrutenkoEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoDeepti Varshney avatar Deepti VarshneyKenji Fujihara avatar Kenji Fujiharaorcid logoFelicitas Kindel avatar Felicitas KindelBazante Sanders avatar Bazante Sanders

Funding

These individuals or organisations provided funding support for the development of this resource