Assembly

DNA sequence data has become an indispensable tool for Molecular Biology & Evolutionary Biology. Study in these fields now require a genome sequence to work from. We call this a ‘Reference Sequence.’ We need to build a reference for each species. We do this by Genome Assembly. De novo Genome Assembly is the process of reconstructing the original DNA sequence from the fragment reads alone.

Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides () and tutorial () buttons below.
Lesson Slides Hands-on Recordings Input dataset Workflows
An Introduction to Genome Assembly
An introduction to get started in genome assembly and annotation
Assembly of metagenomic sequencing data
assembly metagenomics microgalaxy
Assembly of the mitochondrial genome from PacBio HiFi reads
biodiversity
Chloroplast genome assembly
plants nanopore jbrowse1 biodiversity
De Bruijn Graph Assembly
Decontamination of a genome assembly
biodiversity
Deeper look into Genome Assembly algorithms
ERGA post-assembly QC
plants animals genome assembly QC biodiversity
Genome Assembly Quality Control
assembly quality control biodiversity
Genome Assembly of MRSA from Oxford Nanopore MinION data (and optionally Illumina data)
nanopore assembly amr microgalaxy
Genome Assembly of a bacterial genome (MRSA) sequenced using Illumina MiSeq Data
illumina assembly microgalaxy
Genome assembly and assembly QC - Introduction short version
Genome assembly using PacBio data
assembly pacbio biodiversity
Hybrid genome assembly - Nanopore and Illumina
assembly nanopore illumina denovo genomics
Large genome assembly and polishing
assembly polishing nanopore plants biodiversity
Making sense of a newly assembled genome
gmod prokaryote microgalaxy jbrowse1
Unicycler Assembly
prokaryote microgalaxy biodiversity
Unicycler assembly of SARS-CoV-2 genome with preprocessing to remove human genome reads
covid19 biodiversity
Using the VGP workflows to assemble a vertebrate genome with HiFi and Hi-C data
pacbio eukaryote VGP biodiversity
Vertebrate genome assembly using HiFi, Bionano and Hi-C data - Step by Step
pacbio eukaryote VGP biodiversity

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoTristan Reynolds avatar Tristan ReynoldsAnton Nekrutenko avatar Anton Nekrutenko

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Contributors

This material was contributed to by:

Gildas Le Corguillé avatar Gildas Le Corguilléorcid logoAlexandre Cormier avatar Alexandre CormierEnis Afgan avatar Enis Afganorcid logoNate Coraor avatar Nate Coraororcid logoMiaomiao Zhou avatar Miaomiao ZhouGrace Hall avatar Grace HallMarius van den Beek avatar Marius van den Beekorcid logoHelena Rasche avatar Helena Rascheorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoTom Brown avatar Tom Brownorcid logoLaura Leroi avatar Laura LeroiWilliam Durand avatar William Durandorcid logoLinelle Abueg avatar Linelle Abuegorcid logoAlex Ostrovsky avatar Alex Ostrovskyorcid logoErwan Corre avatar Erwan Correorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoBjörn Grüning avatar Björn GrüningNiall Beard avatar Niall BeardGiulio Formenti avatar Giulio FormentiBrandon Pickett avatar Brandon PickettMarcella Sozzoni avatar Marcella Sozzoniorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoPaul Zierep avatar Paul ZierepMatúš Kalaš avatar Matúš Kalašorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoBérénice Batut avatar Bérénice BatutAnton Nekrutenko avatar Anton Nekrutenkoorcid logoPolina Polunina avatar Polina PoluninaBazante Sanders avatar Bazante Sandersorcid logoSimon Gladman avatar Simon Gladmanorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoVini Salazar avatar Vini Salazarorcid logoAnna Syme avatar Anna Symeorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoSolenne Correard avatar Solenne Correardorcid logoWolfgang Maier avatar Wolfgang MaierVerena Moosmann avatar Verena MoosmannFabian Recktenwald avatar Fabian Recktenwaldorcid logoMaria Doyle avatar Maria Doyleorcid logoTeresa Müller avatar Teresa Müllerorcid logoRomane LIBOUBAN avatar Romane LIBOUBAN

Funding

These individuals or organisations provided funding support for the development of this resource

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Melbourne Bioinformatics
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Australian BioCommons
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Gallantries
This project (2020-1-NL01-KA203-064717) is funded with the support of the Erasmus+ programme of the European Union. Their funding has supported a large number of tutorials within the GTN across a wide array of topics.
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Avans
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ABRomics
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The University of Melbourne