Assembly

DNA sequence data has become an indispensable tool for Molecular Biology & Evolutionary Biology. Study in these fields now require a genome sequence to work from. We call this a ‘Reference Sequence.’ We need to build a reference for each species. We do this by Genome Assembly. De novo Genome Assembly is the process of reconstructing the original DNA sequence from the fragment reads alone.

Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides () and tutorial () buttons below.
Lesson Slides Hands-on Recordings Input dataset Workflows
An Introduction to Genome Assembly
An introduction to get started in genome assembly and annotation
Assembly of metagenomic sequencing data
assembly metagenomics microgalaxy
Assembly of the mitochondrial genome from PacBio HiFi reads
biodiversity
Chloroplast genome assembly
plants nanopore jbrowse1 biodiversity
De Bruijn Graph Assembly
Decontamination of a genome assembly
biodiversity
Deeper look into Genome Assembly algorithms
ERGA post-assembly QC
plants animals genome assembly QC biodiversity
Genome Assembly Quality Control
assembly quality control biodiversity
Genome Assembly of MRSA from Oxford Nanopore MinION data (and optionally Illumina data)
nanopore assembly amr microgalaxy
Genome Assembly of a bacterial genome (MRSA) sequenced using Illumina MiSeq Data
illumina assembly microgalaxy
Genome assembly and assembly QC - Introduction short version
Genome assembly using PacBio data
assembly pacbio biodiversity
Hybrid genome assembly - Nanopore and Illumina
assembly nanopore illumina denovo genomics
Large genome assembly and polishing
assembly polishing nanopore plants biodiversity
Making sense of a newly assembled genome
gmod prokaryote microgalaxy jbrowse1
Unicycler Assembly
prokaryote microgalaxy biodiversity
Unicycler assembly of SARS-CoV-2 genome with preprocessing to remove human genome reads
covid19 biodiversity
Using the VGP workflows to assemble a vertebrate genome with HiFi and Hi-C data
pacbio eukaryote VGP biodiversity
Vertebrate genome assembly using HiFi, Bionano and Hi-C data - Step by Step
pacbio eukaryote VGP biodiversity

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoTristan Reynolds avatar Tristan ReynoldsAnton Nekrutenko avatar Anton Nekrutenko

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Contributors

This material was contributed to by:

Verena Moosmann avatar Verena Moosmannorcid logoSolenne Correard avatar Solenne Correardorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoSimon Gladman avatar Simon Gladmanorcid logoMaria Doyle avatar Maria Doyleorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoVini Salazar avatar Vini Salazarorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoTom Brown avatar Tom Brownorcid logoMiaomiao Zhou avatar Miaomiao ZhouGiulio Formenti avatar Giulio Formentiorcid logoErwan Corre avatar Erwan Correorcid logoHelena Rasche avatar Helena Rascheorcid logoAlexandre Cormier avatar Alexandre CormierBazante Sanders avatar Bazante SandersMatúš Kalaš avatar Matúš Kalašorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoAlex Ostrovsky avatar Alex OstrovskyMarcella Sozzoni avatar Marcella SozzoniNiall Beard avatar Niall Beardorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoNate Coraor avatar Nate Coraororcid logoBjörn Grüning avatar Björn GrüningFabian Recktenwald avatar Fabian Recktenwaldorcid logoPolina Polunina avatar Polina PoluninaBrandon Pickett avatar Brandon PickettGildas Le Corguillé avatar Gildas Le CorguilléGrace Hall avatar Grace Hallorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoLinelle Abueg avatar Linelle Abuegorcid logoWolfgang Maier avatar Wolfgang Maierorcid logoBérénice Batut avatar Bérénice Batutorcid logoLaura Leroi avatar Laura LeroiWilliam Durand avatar William Durandorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoAnthony Bretaudeau avatar Anthony BretaudeauMarius van den Beek avatar Marius van den Beekorcid logoPaul Zierep avatar Paul Ziereporcid logoTeresa Müller avatar Teresa MüllerEnis Afgan avatar Enis AfganAnton Nekrutenko avatar Anton Nekrutenkoorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoAnna Syme avatar Anna Syme

Funding

These individuals or organisations provided funding support for the development of this resource

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Avans
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Australian BioCommons
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The University of Melbourne
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Gallantries
This project (2020-1-NL01-KA203-064717) is funded with the support of the Erasmus+ programme of the European Union. Their funding has supported a large number of tutorials within the GTN across a wide array of topics.
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Melbourne Bioinformatics
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ABRomics