Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Functional profiling of a bacterial genome with Eggsplorer
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoAnika Erxleben avatar Anika Erxlebenorcid logoPaul Zierep avatar Paul Ziereporcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoNicola Soranzo avatar Nicola SoranzoHélène Chiapello avatar Hélène Chiapelloorcid logoLaura Leroi avatar Laura Leroiorcid logoSimon Gladman avatar Simon Gladmanorcid logoSaskia Hiltemann avatar Saskia HiltemannTwishi Gulati avatar Twishi GulatiNiall Beard avatar Niall BeardNathan Dunn avatar Nathan DunnEkaterina Polkh avatar Ekaterina PolkhEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilGildas Le Corguillé avatar Gildas Le Corguilléorcid logoSaim Momin avatar Saim MominTorsten Seemann avatar Torsten SeemannBazante Sanders avatar Bazante Sandersorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoBjörn Grüning avatar Björn Grüningorcid logoHelena Rasche avatar Helena RascheJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoMiaomiao Zhou avatar Miaomiao ZhouDonny Vrins avatar Donny Vrinsorcid logoTeresa Müller avatar Teresa Müllerorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoAnna Syme avatar Anna Symeorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoMaria Doyle avatar Maria Doyleorcid logoDelphine Lariviere avatar Delphine LariviereKenji Fujihara avatar Kenji FujiharaValentin Loux avatar Valentin Louxorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoSolenne Correard avatar Solenne CorreardWilliam Durand avatar William Durandorcid logoVesselin Baev avatar Vesselin BaevSimon Bray avatar Simon Brayorcid logoNiklas Mayle avatar Niklas Mayleorcid logoMateo Boudet avatar Mateo BoudetGuillaume Gautreau avatar Guillaume Gautreauorcid logoStéphanie Robin avatar Stéphanie RobinAnton Nekrutenko avatar Anton Nekrutenkoorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoBérénice Batut avatar Bérénice Batutorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoErwan Corre avatar Erwan Corre

Funding

These individuals or organisations provided funding support for the development of this resource