Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoMaria Doyle avatar Maria Doyleorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoSaskia Hiltemann avatar Saskia HiltemannWilliam Durand avatar William Durandorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoJonathan Kreplak avatar Jonathan KreplakHélène Chiapello avatar Hélène Chiapelloorcid logoMateo Boudet avatar Mateo Boudetorcid logoLaura Leroi avatar Laura LeroiNiall Beard avatar Niall BeardEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoStéphanie Robin avatar Stéphanie RobinGuillaume Gautreau avatar Guillaume Gautreauorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoFelicitas Kindel avatar Felicitas KindelKenji Fujihara avatar Kenji Fujiharaorcid logoBérénice Batut avatar Bérénice Batutorcid logoAnthony Bretaudeau avatar Anthony BretaudeauNathan Dunn avatar Nathan Dunnorcid logoTristan Reynolds avatar Tristan ReynoldsGildas Le Corguillé avatar Gildas Le CorguilléBazante Sanders avatar Bazante SandersTorsten Seemann avatar Torsten SeemannTwishi Gulati avatar Twishi Gulatiorcid logoTeresa Müller avatar Teresa MüllerSimon Bray avatar Simon Brayorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoHelena Rasche avatar Helena RascheEkaterina Polkh avatar Ekaterina Polkhorcid logoBjörn Grüning avatar Björn Grüningorcid logoDelphine Lariviere avatar Delphine LariviereAnton Nekrutenko avatar Anton Nekrutenkoorcid logoSaim Momin avatar Saim Mominorcid logoPaul Zierep avatar Paul Ziereporcid logoSimon Gladman avatar Simon Gladmanorcid logoErwan Corre avatar Erwan Correorcid logoNiklas Mayle avatar Niklas MayleJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoAnna Syme avatar Anna SymeValentin Loux avatar Valentin Louxorcid logoAlexandre Cormier avatar Alexandre Cormier

Funding

These individuals or organisations provided funding support for the development of this resource