Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoStéphanie Robin avatar Stéphanie Robinorcid logoMiaomiao Zhou avatar Miaomiao ZhouNathan Dunn avatar Nathan Dunnorcid logoSimon Gladman avatar Simon GladmanValentin Loux avatar Valentin Louxorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoTeresa Müller avatar Teresa MüllerKenji Fujihara avatar Kenji Fujiharaorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoErwan Corre avatar Erwan Correorcid logoAnna Syme avatar Anna SymeEkaterina Polkh avatar Ekaterina Polkhorcid logoHelena Rasche avatar Helena RascheTorsten Seemann avatar Torsten Seemannorcid logoBjörn Grüning avatar Björn GrüningNiall Beard avatar Niall Beardorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoSaskia Hiltemann avatar Saskia HiltemannHélène Chiapello avatar Hélène Chiapelloorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoMaria Doyle avatar Maria DoyleEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoPaul Zierep avatar Paul Ziereporcid logoLaura Leroi avatar Laura Leroiorcid logoAnika Erxleben avatar Anika ErxlebenSimon Bray avatar Simon Brayorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoBérénice Batut avatar Bérénice BatutGildas Le Corguillé avatar Gildas Le Corguilléorcid logoMateo Boudet avatar Mateo Boudetorcid logoTristan Reynolds avatar Tristan ReynoldsWilliam Durand avatar William DurandBazante Sanders avatar Bazante Sandersorcid logoSaim Momin avatar Saim Mominorcid logoDeepti Varshney avatar Deepti VarshneyTwishi Gulati avatar Twishi GulatiAnton Nekrutenko avatar Anton NekrutenkoGuillaume Gautreau avatar Guillaume Gautreauorcid logoNiklas Mayle avatar Niklas MayleJasper Ouwerkerk avatar Jasper Ouwerkerk

Funding

These individuals or organisations provided funding support for the development of this resource