Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Esteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoMiaomiao Zhou avatar Miaomiao ZhouAnton Nekrutenko avatar Anton Nekrutenkoorcid logoNiklas Mayle avatar Niklas Mayleorcid logoBjörn Grüning avatar Björn GrüningTorsten Seemann avatar Torsten Seemannorcid logoDeepti Varshney avatar Deepti VarshneyTwishi Gulati avatar Twishi GulatiGuillaume Gautreau avatar Guillaume Gautreauorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoTeresa Müller avatar Teresa Müllerorcid logoMaria Doyle avatar Maria DoyleEkaterina Polkh avatar Ekaterina Polkhorcid logoSaskia Hiltemann avatar Saskia HiltemannWilliam Durand avatar William Durandorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoRomane LIBOUBAN avatar Romane LIBOUBANJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoLaura Leroi avatar Laura LeroiValentin Loux avatar Valentin Louxorcid logoAnna Syme avatar Anna Symeorcid logoHelena Rasche avatar Helena Rascheorcid logoMateo Boudet avatar Mateo BoudetKenji Fujihara avatar Kenji Fujiharaorcid logoBérénice Batut avatar Bérénice Batutorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoPaul Zierep avatar Paul Ziereporcid logoTristan Reynolds avatar Tristan ReynoldsGildas Le Corguillé avatar Gildas Le CorguilléNiall Beard avatar Niall Beardorcid logoErwan Corre avatar Erwan Correorcid logoCristóbal Gallardo avatar Cristóbal GallardoBazante Sanders avatar Bazante Sandersorcid logoSaim Momin avatar Saim Mominorcid logoSimon Gladman avatar Simon GladmanSimon Bray avatar Simon Brayorcid logoAnthony Bretaudeau avatar Anthony BretaudeauHélène Chiapello avatar Hélène Chiapelloorcid logoAnika Erxleben avatar Anika ErxlebenNathan Dunn avatar Nathan Dunnorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoFelicitas Kindel avatar Felicitas Kindel

Funding

These individuals or organisations provided funding support for the development of this resource