Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoCristóbal Gallardo avatar Cristóbal GallardoSimon Bray avatar Simon BrayEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoBérénice Batut avatar Bérénice Batutorcid logoPaul Zierep avatar Paul Ziereporcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoHelena Rasche avatar Helena Rascheorcid logoBjörn Grüning avatar Björn Grüningorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoAnna Syme avatar Anna SymeTorsten Seemann avatar Torsten Seemannorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoAnika Erxleben avatar Anika ErxlebenAnton Nekrutenko avatar Anton NekrutenkoEkaterina Polkh avatar Ekaterina Polkhorcid logoMateo Boudet avatar Mateo Boudetorcid logoDelphine Lariviere avatar Delphine LariviereWilliam Durand avatar William DurandHélène Chiapello avatar Hélène Chiapelloorcid logoFelicitas Kindel avatar Felicitas KindelKenji Fujihara avatar Kenji Fujiharaorcid logoErwan Corre avatar Erwan CorreGildas Le Corguillé avatar Gildas Le Corguilléorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoMaria Doyle avatar Maria Doyleorcid logoTeresa Müller avatar Teresa Müllerorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoJonathan Kreplak avatar Jonathan KreplakNiall Beard avatar Niall Beardorcid logoLaura Leroi avatar Laura LeroiValentin Loux avatar Valentin LouxBazante Sanders avatar Bazante Sandersorcid logoSimon Gladman avatar Simon Gladmanorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoNicola Soranzo avatar Nicola SoranzoTwishi Gulati avatar Twishi Gulatiorcid logoDeepti Varshney avatar Deepti VarshneyGuillaume Gautreau avatar Guillaume GautreauNathan Dunn avatar Nathan DunnJasper Ouwerkerk avatar Jasper Ouwerkerk

Funding

These individuals or organisations provided funding support for the development of this resource