Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Pangenome analysis with Roary
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Simon Bray avatar Simon Brayorcid logoAlexandre Cormier avatar Alexandre CormierEkaterina Polkh avatar Ekaterina PolkhEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoNiklas Mayle avatar Niklas Mayleorcid logoMaria Doyle avatar Maria Doyleorcid logoCristóbal Gallardo avatar Cristóbal GallardoAnton Nekrutenko avatar Anton Nekrutenkoorcid logoErwan Corre avatar Erwan Correorcid logoPaul Zierep avatar Paul ZierepNiall Beard avatar Niall Beardorcid logoSimon Gladman avatar Simon Gladmanorcid logoMateo Boudet avatar Mateo Boudetorcid logoStéphanie Robin avatar Stéphanie RobinKenji Fujihara avatar Kenji FujiharaHélène Chiapello avatar Hélène Chiapelloorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoLaura Leroi avatar Laura Leroiorcid logoHelena Rasche avatar Helena Rascheorcid logoSaskia Hiltemann avatar Saskia HiltemannWilliam Durand avatar William DurandTwishi Gulati avatar Twishi GulatiTorsten Seemann avatar Torsten SeemannNathan Dunn avatar Nathan Dunnorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoBjörn Grüning avatar Björn Grüningorcid logoRomane LIBOUBAN avatar Romane LIBOUBANGuillaume Gautreau avatar Guillaume Gautreauorcid logoTeresa Müller avatar Teresa MüllerJasper Ouwerkerk avatar Jasper OuwerkerkValentin Loux avatar Valentin Louxorcid logoAnna Syme avatar Anna Symeorcid logoDeepti Varshney avatar Deepti VarshneyBazante Sanders avatar Bazante Sandersorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoBérénice Batut avatar Bérénice BatutGildas Le Corguillé avatar Gildas Le Corguilléorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoSaim Momin avatar Saim Momin

Funding

These individuals or organisations provided funding support for the development of this resource