Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Bazante Sanders avatar Bazante Sandersorcid logoSimon Gladman avatar Simon GladmanWilliam Durand avatar William Durandorcid logoRomane LIBOUBAN avatar Romane LIBOUBANSimon Bray avatar Simon Brayorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoHelena Rasche avatar Helena Rascheorcid logoAnika Erxleben avatar Anika ErxlebenHélène Chiapello avatar Hélène Chiapelloorcid logoBjörn Grüning avatar Björn Grüningorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoAnthony Bretaudeau avatar Anthony BretaudeauKenji Fujihara avatar Kenji FujiharaValentin Loux avatar Valentin Louxorcid logoPaul Zierep avatar Paul Ziereporcid logoBérénice Batut avatar Bérénice Batutorcid logoAnna Syme avatar Anna SymeEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoMaria Doyle avatar Maria Doyleorcid logoTristan Reynolds avatar Tristan ReynoldsGildas Le Corguillé avatar Gildas Le Corguilléorcid logoLaura Leroi avatar Laura Leroiorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoErwan Corre avatar Erwan Correorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoDelphine Lariviere avatar Delphine LariviereJasper Ouwerkerk avatar Jasper OuwerkerkNathan Dunn avatar Nathan Dunnorcid logoMateo Boudet avatar Mateo BoudetEkaterina Polkh avatar Ekaterina PolkhNiall Beard avatar Niall BeardTwishi Gulati avatar Twishi GulatiTorsten Seemann avatar Torsten Seemannorcid logoDeepti Varshney avatar Deepti VarshneyAnton Nekrutenko avatar Anton Nekrutenkoorcid logoSaskia Hiltemann avatar Saskia HiltemannGuillaume Gautreau avatar Guillaume Gautreauorcid logoTeresa Müller avatar Teresa Müllerorcid logoFelicitas Kindel avatar Felicitas Kindel

Funding

These individuals or organisations provided funding support for the development of this resource