Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoBjörn Grüning avatar Björn Grüningorcid logoNiklas Mayle avatar Niklas Mayleorcid logoMaria Doyle avatar Maria DoyleGuillaume Gautreau avatar Guillaume GautreauValentin Loux avatar Valentin LouxKenji Fujihara avatar Kenji Fujiharaorcid logoLaura Leroi avatar Laura Leroiorcid logoJonathan Kreplak avatar Jonathan KreplakHélène Chiapello avatar Hélène ChiapelloNiall Beard avatar Niall Beardorcid logoPaul Zierep avatar Paul Ziereporcid logoMateo Boudet avatar Mateo Boudetorcid logoAnna Syme avatar Anna SymeBazante Sanders avatar Bazante Sandersorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoDeepti Varshney avatar Deepti VarshneyGildas Le Corguillé avatar Gildas Le Corguilléorcid logoAlexandre Cormier avatar Alexandre CormierEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoSaim Momin avatar Saim MominEkaterina Polkh avatar Ekaterina Polkhorcid logoHelena Rasche avatar Helena RascheTorsten Seemann avatar Torsten SeemannWilliam Durand avatar William Durandorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoBérénice Batut avatar Bérénice Batutorcid logoAnika Erxleben avatar Anika ErxlebenAnton Nekrutenko avatar Anton Nekrutenkoorcid logoStéphanie Robin avatar Stéphanie RobinNathan Dunn avatar Nathan DunnTwishi Gulati avatar Twishi Gulatiorcid logoTeresa Müller avatar Teresa Müllerorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoSimon Gladman avatar Simon Gladmanorcid logoErwan Corre avatar Erwan Correorcid logoCristóbal Gallardo avatar Cristóbal GallardoJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoFelicitas Kindel avatar Felicitas KindelSimon Bray avatar Simon Bray

Funding

These individuals or organisations provided funding support for the development of this resource