Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Functional profiling of a bacterial genome with Eggsplorer
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

Guillaume Gautreau avatar Guillaume Gautreauorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoPaul Zierep avatar Paul Ziereporcid logoHelena Rasche avatar Helena RascheNiall Beard avatar Niall Beardorcid logoTeresa Müller avatar Teresa MüllerKenji Fujihara avatar Kenji Fujiharaorcid logoVesselin Baev avatar Vesselin Baevorcid logoSaim Momin avatar Saim Mominorcid logoSolenne Correard avatar Solenne Correardorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoNiklas Mayle avatar Niklas Mayleorcid logoLaura Leroi avatar Laura Leroiorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoRomane LIBOUBAN avatar Romane LIBOUBANValentin Loux avatar Valentin LouxWilliam Durand avatar William Durandorcid logoAnna Syme avatar Anna SymeNathan Dunn avatar Nathan Dunnorcid logoAnika Erxleben avatar Anika ErxlebenJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoErwan Corre avatar Erwan Correorcid logoStéphanie Robin avatar Stéphanie RobinTorsten Seemann avatar Torsten Seemannorcid logoMaria Doyle avatar Maria Doyleorcid logoBjörn Grüning avatar Björn Grüningorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoFelicitas Kindel avatar Felicitas KindelSimon Bray avatar Simon Brayorcid logoSimon Gladman avatar Simon Gladmanorcid logoTristan Reynolds avatar Tristan ReynoldsBazante Sanders avatar Bazante SandersTwishi Gulati avatar Twishi Gulatiorcid logoDelphine Lariviere avatar Delphine LariviereDonny Vrins avatar Donny Vrinsorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoMateo Boudet avatar Mateo BoudetGildas Le Corguillé avatar Gildas Le Corguilléorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoBérénice Batut avatar Bérénice Batutorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoNicola Soranzo avatar Nicola SoranzoEkaterina Polkh avatar Ekaterina PolkhHélène Chiapello avatar Hélène ChiapelloAnton Nekrutenko avatar Anton NekrutenkoEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeil

Funding

These individuals or organisations provided funding support for the development of this resource