Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Functional profiling of a bacterial genome with Eggsplorer
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoSolenne Correard avatar Solenne CorreardSimon Bray avatar Simon Brayorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoAnna Syme avatar Anna SymeTorsten Seemann avatar Torsten SeemannAnton Nekrutenko avatar Anton Nekrutenkoorcid logoMaria Doyle avatar Maria Doyleorcid logoNiklas Mayle avatar Niklas MayleGildas Le Corguillé avatar Gildas Le Corguilléorcid logoErwan Corre avatar Erwan CorreDonny Vrins avatar Donny Vrinsorcid logoMateo Boudet avatar Mateo BoudetEkaterina Polkh avatar Ekaterina Polkhorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoVesselin Baev avatar Vesselin Baevorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoPaul Zierep avatar Paul Ziereporcid logoLaura Leroi avatar Laura Leroiorcid logoDelphine Lariviere avatar Delphine LariviereKenji Fujihara avatar Kenji Fujiharaorcid logoTristan Reynolds avatar Tristan ReynoldsWilliam Durand avatar William DurandNiall Beard avatar Niall BeardEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilBazante Sanders avatar Bazante Sandersorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoSaskia Hiltemann avatar Saskia HiltemannValentin Loux avatar Valentin Louxorcid logoCristóbal Gallardo avatar Cristóbal GallardoHélène Chiapello avatar Hélène Chiapelloorcid logoBjörn Grüning avatar Björn Grüningorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoSimon Gladman avatar Simon Gladmanorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoBérénice Batut avatar Bérénice BatutTwishi Gulati avatar Twishi Gulatiorcid logoAnika Erxleben avatar Anika ErxlebenNathan Dunn avatar Nathan DunnGuillaume Gautreau avatar Guillaume Gautreauorcid logoHelena Rasche avatar Helena Rascheorcid logoTeresa Müller avatar Teresa Müllerorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoSaim Momin avatar Saim MominJasper Ouwerkerk avatar Jasper Ouwerkerk

Funding

These individuals or organisations provided funding support for the development of this resource