Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoDeepti Varshney avatar Deepti Varshneyorcid logoNiklas Mayle avatar Niklas Mayleorcid logoAnna Syme avatar Anna Symeorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoSimon Gladman avatar Simon GladmanEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilValentin Loux avatar Valentin Louxorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoPaul Zierep avatar Paul ZierepTwishi Gulati avatar Twishi Gulatiorcid logoSaskia Hiltemann avatar Saskia HiltemannWilliam Durand avatar William DurandHélène Chiapello avatar Hélène ChiapelloJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoTeresa Müller avatar Teresa MüllerEkaterina Polkh avatar Ekaterina PolkhBazante Sanders avatar Bazante SandersNiall Beard avatar Niall BeardTorsten Seemann avatar Torsten SeemannKenji Fujihara avatar Kenji Fujiharaorcid logoSaim Momin avatar Saim Mominorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoRomane LIBOUBAN avatar Romane LIBOUBANGildas Le Corguillé avatar Gildas Le Corguilléorcid logoBjörn Grüning avatar Björn GrüningAnton Nekrutenko avatar Anton Nekrutenkoorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoBérénice Batut avatar Bérénice Batutorcid logoLaura Leroi avatar Laura LeroiSimon Bray avatar Simon Brayorcid logoErwan Corre avatar Erwan Correorcid logoNicola Soranzo avatar Nicola SoranzoGuillaume Gautreau avatar Guillaume Gautreauorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoMaria Doyle avatar Maria Doyleorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoMateo Boudet avatar Mateo BoudetNathan Dunn avatar Nathan Dunnorcid logoHelena Rasche avatar Helena Rascheorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoDelphine Lariviere avatar Delphine Lariviere

Funding

These individuals or organisations provided funding support for the development of this resource