Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoNicola Soranzo avatar Nicola Soranzoorcid logoErwan Corre avatar Erwan Correorcid logoBjörn Grüning avatar Björn GrüningSimon Bray avatar Simon BrayTwishi Gulati avatar Twishi Gulatiorcid logoAnika Erxleben avatar Anika ErxlebenJasper Ouwerkerk avatar Jasper OuwerkerkAnton Nekrutenko avatar Anton Nekrutenkoorcid logoTeresa Müller avatar Teresa Müllerorcid logoMaria Doyle avatar Maria Doyleorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoSaskia Hiltemann avatar Saskia HiltemannGuillaume Gautreau avatar Guillaume GautreauNiall Beard avatar Niall Beardorcid logoMateo Boudet avatar Mateo Boudetorcid logoAnthony Bretaudeau avatar Anthony BretaudeauBazante Sanders avatar Bazante SandersTorsten Seemann avatar Torsten Seemannorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoSimon Gladman avatar Simon Gladmanorcid logoJonathan Kreplak avatar Jonathan KreplakHélène Chiapello avatar Hélène Chiapelloorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoBérénice Batut avatar Bérénice BatutNathan Dunn avatar Nathan Dunnorcid logoTristan Reynolds avatar Tristan ReynoldsWilliam Durand avatar William Durandorcid logoMiaomiao Zhou avatar Miaomiao ZhouEkaterina Polkh avatar Ekaterina Polkhorcid logoAnna Syme avatar Anna Symeorcid logoSaim Momin avatar Saim Mominorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoPaul Zierep avatar Paul ZierepEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilValentin Loux avatar Valentin LouxGildas Le Corguillé avatar Gildas Le Corguilléorcid logoNiklas Mayle avatar Niklas Mayleorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoLaura Leroi avatar Laura Leroiorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoDeepti Varshney avatar Deepti VarshneyKenji Fujihara avatar Kenji Fujiharaorcid logoHelena Rasche avatar Helena Rasche

Funding

These individuals or organisations provided funding support for the development of this resource