Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoBérénice Batut avatar Bérénice Batutorcid logoRomane LIBOUBAN avatar Romane LIBOUBANHélène Chiapello avatar Hélène Chiapelloorcid logoErwan Corre avatar Erwan CorreAnton Nekrutenko avatar Anton Nekrutenkoorcid logoDelphine Lariviere avatar Delphine LariviereEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoFelicitas Kindel avatar Felicitas KindelEkaterina Polkh avatar Ekaterina Polkhorcid logoAnna Syme avatar Anna Symeorcid logoSimon Gladman avatar Simon GladmanWilliam Durand avatar William Durandorcid logoBjörn Grüning avatar Björn GrüningSimon Bray avatar Simon BrayNiall Beard avatar Niall BeardGuillaume Gautreau avatar Guillaume GautreauNathan Dunn avatar Nathan Dunnorcid logoLaura Leroi avatar Laura Leroiorcid logoMaria Doyle avatar Maria DoyleKenji Fujihara avatar Kenji Fujiharaorcid logoCristóbal Gallardo avatar Cristóbal GallardoTorsten Seemann avatar Torsten SeemannBazante Sanders avatar Bazante Sandersorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoStéphanie Robin avatar Stéphanie RobinValentin Loux avatar Valentin Louxorcid logoTeresa Müller avatar Teresa MüllerTwishi Gulati avatar Twishi Gulatiorcid logoPaul Zierep avatar Paul Ziereporcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoSaskia Hiltemann avatar Saskia HiltemannJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoJonathan Kreplak avatar Jonathan KreplakGildas Le Corguillé avatar Gildas Le Corguilléorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoMateo Boudet avatar Mateo Boudetorcid logoHelena Rasche avatar Helena Rasche

Funding

These individuals or organisations provided funding support for the development of this resource