Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

For any question or discussions related to this topic, or to connect with others in the community, please visit the community chat:

comment   Community Matrix Chat

Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

Follow topic updates rss-feed with our RSS Feed

Community Resources

Community Home Maintainer Home

Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

Are you on the editorial board or want to help out with this topic? View the topic maintainer page for ways to improve this topic!

Contributors

This material was contributed to by:

orcid logoCristóbal Gallardo avatar Cristóbal GallardoGuillaume Gautreau avatar Guillaume GautreauValentin Loux avatar Valentin Louxorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoTeresa Müller avatar Teresa Müllerorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoBérénice Batut avatar Bérénice BatutSimon Bray avatar Simon BrayEkaterina Polkh avatar Ekaterina Polkhorcid logoHelena Rasche avatar Helena Rascheorcid logoPaul Zierep avatar Paul ZierepEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoBjörn Grüning avatar Björn Grüningorcid logoMiaomiao Zhou avatar Miaomiao ZhouNathan Dunn avatar Nathan Dunnorcid logoMateo Boudet avatar Mateo BoudetKenji Fujihara avatar Kenji Fujiharaorcid logoNicola Soranzo avatar Nicola SoranzoAnton Nekrutenko avatar Anton NekrutenkoTwishi Gulati avatar Twishi GulatiHélène Chiapello avatar Hélène Chiapelloorcid logoStéphanie Robin avatar Stéphanie RobinWilliam Durand avatar William Durandorcid logoDeepti Varshney avatar Deepti VarshneyGildas Le Corguillé avatar Gildas Le Corguilléorcid logoAnthony Bretaudeau avatar Anthony BretaudeauNiall Beard avatar Niall BeardBazante Sanders avatar Bazante Sandersorcid logoSimon Gladman avatar Simon GladmanJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoLaura Leroi avatar Laura Leroiorcid logoErwan Corre avatar Erwan Correorcid logoJonathan Kreplak avatar Jonathan KreplakTorsten Seemann avatar Torsten Seemannorcid logoMaria Doyle avatar Maria Doyleorcid logoAnna Syme avatar Anna Syme

Funding

These individuals or organisations provided funding support for the development of this resource