Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoHelena Rasche avatar Helena Rascheorcid logoSaim Momin avatar Saim Mominorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoMaria Doyle avatar Maria DoyleJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoPaul Zierep avatar Paul Ziereporcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoTeresa Müller avatar Teresa MüllerTwishi Gulati avatar Twishi Gulatiorcid logoMiaomiao Zhou avatar Miaomiao ZhouWilliam Durand avatar William DurandAnton Nekrutenko avatar Anton Nekrutenkoorcid logoLaura Leroi avatar Laura Leroiorcid logoAnika Erxleben avatar Anika ErxlebenNathan Dunn avatar Nathan DunnEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoBjörn Grüning avatar Björn GrüningValentin Loux avatar Valentin Louxorcid logoSimon Gladman avatar Simon GladmanEkaterina Polkh avatar Ekaterina PolkhSimon Bray avatar Simon Brayorcid logoTristan Reynolds avatar Tristan ReynoldsGuillaume Gautreau avatar Guillaume Gautreauorcid logoAnthony Bretaudeau avatar Anthony BretaudeauGildas Le Corguillé avatar Gildas Le Corguilléorcid logoNiklas Mayle avatar Niklas Mayleorcid logoBérénice Batut avatar Bérénice Batutorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoAnna Syme avatar Anna SymeHélène Chiapello avatar Hélène Chiapelloorcid logoAlexandre Cormier avatar Alexandre CormierTorsten Seemann avatar Torsten SeemannNiall Beard avatar Niall Beardorcid logoErwan Corre avatar Erwan CorreKenji Fujihara avatar Kenji FujiharaBazante Sanders avatar Bazante Sandersorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoMateo Boudet avatar Mateo Boudet

Funding

These individuals or organisations provided funding support for the development of this resource