Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides () and tutorial () buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
bacteria tnseq essential genes microgalaxy
Bacterial Genome Annotation
gmod illumina bacteria microgalaxy jbrowse1
Genome annotation with Prokka
gmod prokaryote microgalaxy jbrowse1
Identification of AMR genes in an assembled bacterial genome
gmod illumina amr one-health jbrowse1 microgalaxy
Refining Genome Annotations with Apollo (prokaryotes)
gmod prokaryote microgalaxy jbrowse1 biodiversity apollo2

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
eukaryote biodiversity
Genome annotation with Funannotate
gmod eukaryote jbrowse1 biodiversity
Genome annotation with Maker (short)
gmod eukaryote maker jbrowse1
Genome annotation with Maker
gmod eukaryote jbrowse1 maker
Functional annotation of protein sequences
eukaryote biodiversity
Refining Genome Annotations with Apollo (eukaryotes)
gmod eukaryote cyoa jbrowse1 apollo2 biodiversity
Long non-coding RNAs (lncRNAs) annotation with FEELnc
eukaryote
CRISPR screen analysis
genome editing CRISPR essential genes cell line
Comparison of two annotation tools - Helixer and Braker3
eukaryota Braker3 Helixer jbrowse1 biodiversity
Genome annotation with Braker3
eukaryota Braker3 jbrowse1 biodiversity
Genome annotation with Helixer
eukaryota helixer jbrowse1 biodiversity

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
evolution annotation vgp cookbook eukaryote prokaryote microgalaxy biodiversity
From small to large-scale genome comparison
plants prokaryote
Pangenome analysis with Roary
pangenome prokaryote phylogeny

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Simon Bray avatar Simon Brayorcid logoBjörn Grüning avatar Björn Grüningorcid logoErwan Corre avatar Erwan Correorcid logoBérénice Batut avatar Bérénice Batutorcid logoMaria Doyle avatar Maria DoyleValentin Loux avatar Valentin LouxHélène Chiapello avatar Hélène ChiapelloNiall Beard avatar Niall Beardorcid logoLaura Leroi avatar Laura LeroiJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoMateo Boudet avatar Mateo Boudetorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoSimon Gladman avatar Simon Gladmanorcid logoHelena Rasche avatar Helena RascheNathan Dunn avatar Nathan DunnWilliam Durand avatar William Durandorcid logoJonathan Kreplak avatar Jonathan KreplakTorsten Seemann avatar Torsten SeemannEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoNiklas Mayle avatar Niklas Mayleorcid logoAnna Syme avatar Anna Symeorcid logoTristan Reynolds avatar Tristan ReynoldsAnton Nekrutenko avatar Anton NekrutenkoGuillaume Gautreau avatar Guillaume Gautreauorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoAnika Erxleben avatar Anika ErxlebenBazante Sanders avatar Bazante Sandersorcid logoCristóbal Gallardo avatar Cristóbal GallardoGildas Le Corguillé avatar Gildas Le Corguilléorcid logoPaul Zierep avatar Paul ZierepKenji Fujihara avatar Kenji Fujiharaorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoSaim Momin avatar Saim MominEkaterina Polkh avatar Ekaterina Polkhorcid logoTeresa Müller avatar Teresa MüllerTwishi Gulati avatar Twishi Gulatiorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoSaskia Hiltemann avatar Saskia Hiltemann

Funding

These individuals or organisations provided funding support for the development of this resource

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Melbourne Bioinformatics
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Avans
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IFB
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EuroScienceGateway
EuroScienceGateway was funded by the European Union programme Horizon Europe (HORIZON-INFRA-2021-EOSC-01-04) under grant agreement number 101057388 and by UK Research and Innovation (UKRI) under the UK government’s Horizon Europe funding guarantee grant number 10038963.
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Australian BioCommons
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ABRomics
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ELIXIR Europe
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The University of Melbourne
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Gallantries
This project (2020-1-NL01-KA203-064717) is funded with the support of the Erasmus+ programme of the European Union. Their funding has supported a large number of tutorials within the GTN across a wide array of topics.