Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoMaria Doyle avatar Maria Doyleorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoBérénice Batut avatar Bérénice BatutEkaterina Polkh avatar Ekaterina Polkhorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoSimon Gladman avatar Simon Gladmanorcid logoNicola Soranzo avatar Nicola SoranzoJasper Ouwerkerk avatar Jasper OuwerkerkHélène Chiapello avatar Hélène Chiapelloorcid logoPaul Zierep avatar Paul Ziereporcid logoAnika Erxleben avatar Anika ErxlebenTorsten Seemann avatar Torsten SeemannAnton Nekrutenko avatar Anton Nekrutenkoorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoDelphine Lariviere avatar Delphine LariviereTwishi Gulati avatar Twishi GulatiWilliam Durand avatar William Durandorcid logoTeresa Müller avatar Teresa MüllerNiall Beard avatar Niall BeardKenji Fujihara avatar Kenji Fujiharaorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoAnna Syme avatar Anna Symeorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony BretaudeauValentin Loux avatar Valentin Louxorcid logoNiklas Mayle avatar Niklas Mayleorcid logoMateo Boudet avatar Mateo BoudetGuillaume Gautreau avatar Guillaume Gautreauorcid logoErwan Corre avatar Erwan CorreBazante Sanders avatar Bazante Sandersorcid logoBjörn Grüning avatar Björn GrüningGildas Le Corguillé avatar Gildas Le Corguilléorcid logoLaura Leroi avatar Laura Leroiorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoSaim Momin avatar Saim MominSimon Bray avatar Simon BrayNathan Dunn avatar Nathan DunnEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeil

Funding

These individuals or organisations provided funding support for the development of this resource