Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

For any question or discussions related to this topic, or to connect with others in the community, please visit the community chat:

comment   Community Matrix Chat

Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Pangenome analysis with Roary
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

Follow topic updates rss-feed with our RSS Feed

Community Resources

Community Home Maintainer Home

Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

Are you on the editorial board or want to help out with this topic? View the topic maintainer page for ways to improve this topic!

Contributors

This material was contributed to by:

orcid logoAnna Syme avatar Anna SymeHélène Chiapello avatar Hélène Chiapelloorcid logoLaura Leroi avatar Laura Leroiorcid logoMaria Doyle avatar Maria Doyleorcid logoErwan Corre avatar Erwan Correorcid logoTeresa Müller avatar Teresa MüllerNiall Beard avatar Niall Beardorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoBérénice Batut avatar Bérénice BatutTorsten Seemann avatar Torsten Seemannorcid logoDelphine Lariviere avatar Delphine LariviereBazante Sanders avatar Bazante Sandersorcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoMateo Boudet avatar Mateo Boudetorcid logoNiklas Mayle avatar Niklas Mayleorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoBjörn Grüning avatar Björn GrüningGuillaume Gautreau avatar Guillaume GautreauWilliam Durand avatar William DurandAnton Nekrutenko avatar Anton NekrutenkoGildas Le Corguillé avatar Gildas Le CorguilléSimon Bray avatar Simon BrayNathan Dunn avatar Nathan Dunnorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoSaim Momin avatar Saim Mominorcid logoAlexandre Cormier avatar Alexandre CormierEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoSimon Gladman avatar Simon GladmanTwishi Gulati avatar Twishi Gulatiorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoJonathan Kreplak avatar Jonathan KreplakValentin Loux avatar Valentin LouxJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoPaul Zierep avatar Paul Ziereporcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoAnika Erxleben avatar Anika ErxlebenKenji Fujihara avatar Kenji Fujiharaorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoDeepti Varshney avatar Deepti VarshneyEkaterina Polkh avatar Ekaterina Polkhorcid logoRomane LIBOUBAN avatar Romane LIBOUBAN

Funding

These individuals or organisations provided funding support for the development of this resource