Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoPaul Zierep avatar Paul Ziereporcid logoHelena Rasche avatar Helena RascheGildas Le Corguillé avatar Gildas Le Corguilléorcid logoAnna Syme avatar Anna SymeKenji Fujihara avatar Kenji Fujiharaorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoCristóbal Gallardo avatar Cristóbal GallardoHélène Chiapello avatar Hélène Chiapelloorcid logoMaria Doyle avatar Maria DoyleTwishi Gulati avatar Twishi Gulatiorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoSaim Momin avatar Saim MominAnton Nekrutenko avatar Anton NekrutenkoNathan Dunn avatar Nathan Dunnorcid logoSimon Gladman avatar Simon Gladmanorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoBjörn Grüning avatar Björn Grüningorcid logoErwan Corre avatar Erwan Correorcid logoAnthony Bretaudeau avatar Anthony BretaudeauJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoStéphanie Robin avatar Stéphanie RobinEkaterina Polkh avatar Ekaterina Polkhorcid logoLaura Leroi avatar Laura LeroiValentin Loux avatar Valentin LouxTorsten Seemann avatar Torsten Seemannorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoBérénice Batut avatar Bérénice BatutGuillaume Gautreau avatar Guillaume Gautreauorcid logoTeresa Müller avatar Teresa MüllerWilliam Durand avatar William Durandorcid logoMateo Boudet avatar Mateo Boudetorcid logoNiklas Mayle avatar Niklas Mayleorcid logoAnika Erxleben avatar Anika ErxlebenNiall Beard avatar Niall Beardorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoAlexandre Cormier avatar Alexandre CormierSimon Bray avatar Simon Brayorcid logoNicola Soranzo avatar Nicola SoranzoBazante Sanders avatar Bazante Sandersorcid logoDeepti Varshney avatar Deepti VarshneyEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoTristan Reynolds avatar Tristan Reynolds

Funding

These individuals or organisations provided funding support for the development of this resource