Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoMaria Doyle avatar Maria Doyleorcid logoMiaomiao Zhou avatar Miaomiao ZhouGildas Le Corguillé avatar Gildas Le Corguilléorcid logoAnna Syme avatar Anna Symeorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoBjörn Grüning avatar Björn Grüningorcid logoLaura Leroi avatar Laura Leroiorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoMateo Boudet avatar Mateo Boudetorcid logoDelphine Lariviere avatar Delphine LariviereValentin Loux avatar Valentin LouxTorsten Seemann avatar Torsten Seemannorcid logoJonathan Kreplak avatar Jonathan KreplakKenji Fujihara avatar Kenji FujiharaWilliam Durand avatar William Durandorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoSaim Momin avatar Saim Mominorcid logoTeresa Müller avatar Teresa Müllerorcid logoBérénice Batut avatar Bérénice BatutAnton Nekrutenko avatar Anton Nekrutenkoorcid logoPaul Zierep avatar Paul Ziereporcid logoRomane LIBOUBAN avatar Romane LIBOUBANTwishi Gulati avatar Twishi Gulatiorcid logoSimon Gladman avatar Simon Gladmanorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoNiklas Mayle avatar Niklas MayleHélène Chiapello avatar Hélène ChiapelloSimon Bray avatar Simon BrayEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilNathan Dunn avatar Nathan Dunnorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoAnthony Bretaudeau avatar Anthony BretaudeauBazante Sanders avatar Bazante SandersJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoAlexandre Cormier avatar Alexandre CormierNiall Beard avatar Niall Beardorcid logoHelena Rasche avatar Helena Rascheorcid logoErwan Corre avatar Erwan CorreGuillaume Gautreau avatar Guillaume Gautreauorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoSaskia Hiltemann avatar Saskia HiltemannEkaterina Polkh avatar Ekaterina Polkh

Funding

These individuals or organisations provided funding support for the development of this resource