Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Gildas Le Corguillé avatar Gildas Le Corguilléorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoHelena Rasche avatar Helena Rascheorcid logoBjörn Grüning avatar Björn Grüningorcid logoTeresa Müller avatar Teresa Müllerorcid logoPaul Zierep avatar Paul Ziereporcid logoStéphanie Robin avatar Stéphanie RobinEkaterina Polkh avatar Ekaterina PolkhWilliam Durand avatar William DurandBazante Sanders avatar Bazante Sandersorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoLaura Leroi avatar Laura LeroiSimon Bray avatar Simon BrayValentin Loux avatar Valentin Louxorcid logoSimon Gladman avatar Simon GladmanNiall Beard avatar Niall BeardHélène Chiapello avatar Hélène Chiapelloorcid logoAlexandre Cormier avatar Alexandre CormierNathan Dunn avatar Nathan DunnAnton Nekrutenko avatar Anton Nekrutenkoorcid logoJonathan Kreplak avatar Jonathan KreplakKenji Fujihara avatar Kenji FujiharaGuillaume Gautreau avatar Guillaume GautreauTwishi Gulati avatar Twishi Gulatiorcid logoErwan Corre avatar Erwan CorreJasper Ouwerkerk avatar Jasper OuwerkerkEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilTorsten Seemann avatar Torsten Seemannorcid logoMateo Boudet avatar Mateo Boudetorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoAnna Syme avatar Anna Symeorcid logoMaria Doyle avatar Maria Doyleorcid logoBérénice Batut avatar Bérénice Batutorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoRomane LIBOUBAN avatar Romane LIBOUBAN

Funding

These individuals or organisations provided funding support for the development of this resource