Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoLaura Leroi avatar Laura LeroiTwishi Gulati avatar Twishi GulatiKenji Fujihara avatar Kenji Fujiharaorcid logoSaskia Hiltemann avatar Saskia HiltemannNathan Dunn avatar Nathan DunnNiall Beard avatar Niall Beardorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoBérénice Batut avatar Bérénice BatutGuillaume Gautreau avatar Guillaume Gautreauorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoErwan Corre avatar Erwan Correorcid logoPaul Zierep avatar Paul ZierepBazante Sanders avatar Bazante SandersValentin Loux avatar Valentin Louxorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoNiklas Mayle avatar Niklas Mayleorcid logoMaria Doyle avatar Maria Doyleorcid logoTeresa Müller avatar Teresa Müllerorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoJonathan Kreplak avatar Jonathan KreplakTorsten Seemann avatar Torsten Seemannorcid logoHelena Rasche avatar Helena Rascheorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoAlexandre Cormier avatar Alexandre CormierGildas Le Corguillé avatar Gildas Le Corguilléorcid logoAnthony Bretaudeau avatar Anthony BretaudeauEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoStéphanie Robin avatar Stéphanie RobinAnton Nekrutenko avatar Anton Nekrutenkoorcid logoDelphine Lariviere avatar Delphine LariviereSimon Bray avatar Simon BrayJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoBjörn Grüning avatar Björn Grüningorcid logoAnna Syme avatar Anna Symeorcid logoSaim Momin avatar Saim Mominorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoSimon Gladman avatar Simon Gladmanorcid logoMateo Boudet avatar Mateo BoudetHélène Chiapello avatar Hélène ChiapelloWilliam Durand avatar William DurandEkaterina Polkh avatar Ekaterina Polkh

Funding

These individuals or organisations provided funding support for the development of this resource