Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

For any question or discussions related to this topic, or to connect with others in the community, please visit the community chat:

comment   Community Matrix Chat

Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

Follow topic updates rss-feed with our RSS Feed

Community Resources

Community Home Maintainer Home

Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

Are you on the editorial board or want to help out with this topic? View the topic maintainer page for ways to improve this topic!

Contributors

This material was contributed to by:

Esteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilTwishi Gulati avatar Twishi GulatiAnton Nekrutenko avatar Anton Nekrutenkoorcid logoNiklas Mayle avatar Niklas Mayleorcid logoHelena Rasche avatar Helena Rascheorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoSimon Gladman avatar Simon Gladmanorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoErwan Corre avatar Erwan Correorcid logoNicola Soranzo avatar Nicola SoranzoJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoTeresa Müller avatar Teresa Müllerorcid logoMiaomiao Zhou avatar Miaomiao ZhouGildas Le Corguillé avatar Gildas Le CorguilléBazante Sanders avatar Bazante SandersKenji Fujihara avatar Kenji Fujiharaorcid logoAnthony Bretaudeau avatar Anthony BretaudeauTorsten Seemann avatar Torsten Seemannorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoMateo Boudet avatar Mateo Boudetorcid logoPaul Zierep avatar Paul Ziereporcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoBjörn Grüning avatar Björn Grüningorcid logoSaim Momin avatar Saim Mominorcid logoLaura Leroi avatar Laura Leroiorcid logoBérénice Batut avatar Bérénice BatutSimon Bray avatar Simon Brayorcid logoDelphine Lariviere avatar Delphine LariviereEkaterina Polkh avatar Ekaterina PolkhGuillaume Gautreau avatar Guillaume GautreauNiall Beard avatar Niall Beardorcid logoMaria Doyle avatar Maria DoyleValentin Loux avatar Valentin Louxorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoAnna Syme avatar Anna SymeHélène Chiapello avatar Hélène Chiapelloorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoFelicitas Kindel avatar Felicitas KindelWilliam Durand avatar William DurandNathan Dunn avatar Nathan Dunn

Funding

These individuals or organisations provided funding support for the development of this resource