Genome Annotation
Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.
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Requirements
Before diving into this topic, we recommend you to have a look at:
Material
You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.Introduction
Start here if you are new to genome annotation in Galaxy.
| Lesson | Slides | Hands-on | Recordings | Input dataset | Workflows |
|---|---|---|---|---|---|
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Introduction to Genome Annotation
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Prokaryotes
Annotation of prokaryotic genomes.
| Lesson | Slides | Hands-on | Recordings | Input dataset | Workflows |
|---|---|---|---|---|---|
| Essential genes detection with Transposon insertion sequencing | |||||
| Bacterial Genome Annotation | |||||
| Genome annotation with Prokka | |||||
| Identification of AMR genes in an assembled bacterial genome | |||||
| Refining Genome Annotations with Apollo (prokaryotes) |
Eukaryotes
Annotation of eukaryotic genomes.
Other
Assorted Tutorials
| Lesson | Slides | Hands-on | Recordings | Input dataset | Workflows |
|---|---|---|---|---|---|
| Comparative gene analysis in unannotated genomes | |||||
| From small to large-scale genome comparison | |||||
| Pangenome analysis with Roary |
Learning Pathways
Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.Frequently Asked Questions
Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.
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