Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Anton Nekrutenko avatar Anton Nekrutenkoorcid logoHelena Rasche avatar Helena Rascheorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoNiklas Mayle avatar Niklas Mayleorcid logoAnna Syme avatar Anna Symeorcid logoTeresa Müller avatar Teresa Müllerorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoMiaomiao Zhou avatar Miaomiao ZhouNiall Beard avatar Niall BeardValentin Loux avatar Valentin Louxorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoDelphine Lariviere avatar Delphine LariviereGuillaume Gautreau avatar Guillaume GautreauBazante Sanders avatar Bazante SandersNathan Dunn avatar Nathan Dunnorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoLaura Leroi avatar Laura Leroiorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoErwan Corre avatar Erwan Correorcid logoBérénice Batut avatar Bérénice Batutorcid logoSimon Gladman avatar Simon Gladmanorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoAnika Erxleben avatar Anika ErxlebenKenji Fujihara avatar Kenji Fujiharaorcid logoSaim Momin avatar Saim MominHélène Chiapello avatar Hélène ChiapelloTwishi Gulati avatar Twishi Gulatiorcid logoMaria Doyle avatar Maria DoyleEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoBjörn Grüning avatar Björn GrüningJasper Ouwerkerk avatar Jasper OuwerkerkWilliam Durand avatar William Durandorcid logoMateo Boudet avatar Mateo BoudetSimon Bray avatar Simon BrayTorsten Seemann avatar Torsten Seemannorcid logoPaul Zierep avatar Paul ZierepEkaterina Polkh avatar Ekaterina PolkhGildas Le Corguillé avatar Gildas Le Corguillé

Funding

These individuals or organisations provided funding support for the development of this resource