Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Functional profiling of a bacterial genome with Eggsplorer
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoBjörn Grüning avatar Björn GrüningSimon Bray avatar Simon Brayorcid logoPaul Zierep avatar Paul Ziereporcid logoHelena Rasche avatar Helena Rascheorcid logoAnika Erxleben avatar Anika ErxlebenKenji Fujihara avatar Kenji Fujiharaorcid logoBérénice Batut avatar Bérénice BatutNiall Beard avatar Niall Beardorcid logoSolenne Correard avatar Solenne Correardorcid logoMateo Boudet avatar Mateo BoudetAnton Nekrutenko avatar Anton Nekrutenkoorcid logoSaim Momin avatar Saim MominBazante Sanders avatar Bazante Sandersorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoSaskia Hiltemann avatar Saskia HiltemannEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoErwan Corre avatar Erwan CorreTwishi Gulati avatar Twishi Gulatiorcid logoMaria Doyle avatar Maria DoyleGildas Le Corguillé avatar Gildas Le Corguilléorcid logoVesselin Baev avatar Vesselin Baevorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoAnthony Bretaudeau avatar Anthony BretaudeauNathan Dunn avatar Nathan DunnGuillaume Gautreau avatar Guillaume Gautreauorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoAlexandre Cormier avatar Alexandre CormierValentin Loux avatar Valentin LouxEkaterina Polkh avatar Ekaterina PolkhHélène Chiapello avatar Hélène Chiapelloorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoDelphine Lariviere avatar Delphine LariviereTorsten Seemann avatar Torsten SeemannJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoTeresa Müller avatar Teresa Müllerorcid logoSimon Gladman avatar Simon GladmanDonny Vrins avatar Donny Vrinsorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoNiklas Mayle avatar Niklas MayleWilliam Durand avatar William Durandorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoLaura Leroi avatar Laura Leroiorcid logoAnna Syme avatar Anna Syme

Funding

These individuals or organisations provided funding support for the development of this resource