Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoSimon Gladman avatar Simon Gladmanorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoLaura Leroi avatar Laura Leroiorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoAnna Syme avatar Anna Symeorcid logoNicola Soranzo avatar Nicola SoranzoKenji Fujihara avatar Kenji FujiharaJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoAnika Erxleben avatar Anika ErxlebenEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilEkaterina Polkh avatar Ekaterina Polkhorcid logoBérénice Batut avatar Bérénice BatutTwishi Gulati avatar Twishi Gulatiorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoHelena Rasche avatar Helena Rascheorcid logoSaskia Hiltemann avatar Saskia HiltemannAnton Nekrutenko avatar Anton Nekrutenkoorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoJonathan Kreplak avatar Jonathan KreplakHélène Chiapello avatar Hélène Chiapelloorcid logoTeresa Müller avatar Teresa MüllerNathan Dunn avatar Nathan Dunnorcid logoBjörn Grüning avatar Björn GrüningSimon Bray avatar Simon Brayorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoPaul Zierep avatar Paul ZierepWilliam Durand avatar William Durandorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoErwan Corre avatar Erwan CorreGildas Le Corguillé avatar Gildas Le Corguilléorcid logoDeepti Varshney avatar Deepti VarshneyNiall Beard avatar Niall Beardorcid logoDelphine Lariviere avatar Delphine LariviereValentin Loux avatar Valentin Louxorcid logoMaria Doyle avatar Maria DoyleGuillaume Gautreau avatar Guillaume GautreauTorsten Seemann avatar Torsten SeemannBazante Sanders avatar Bazante Sandersorcid logoMateo Boudet avatar Mateo Boudet

Funding

These individuals or organisations provided funding support for the development of this resource