Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Niall Beard avatar Niall Beardorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoStéphanie Robin avatar Stéphanie RobinEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoNiklas Mayle avatar Niklas Mayleorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoPaul Zierep avatar Paul Ziereporcid logoBjörn Grüning avatar Björn GrüningEkaterina Polkh avatar Ekaterina PolkhGuillaume Gautreau avatar Guillaume Gautreauorcid logoAnna Syme avatar Anna Symeorcid logoDeepti Varshney avatar Deepti VarshneyTorsten Seemann avatar Torsten SeemannAnton Nekrutenko avatar Anton NekrutenkoGildas Le Corguillé avatar Gildas Le CorguilléHélène Chiapello avatar Hélène Chiapelloorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoSimon Gladman avatar Simon Gladmanorcid logoLaura Leroi avatar Laura Leroiorcid logoMateo Boudet avatar Mateo Boudetorcid logoFelicitas Kindel avatar Felicitas KindelBazante Sanders avatar Bazante Sandersorcid logoSaim Momin avatar Saim Mominorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoBérénice Batut avatar Bérénice Batutorcid logoTeresa Müller avatar Teresa MüllerWilliam Durand avatar William DurandValentin Loux avatar Valentin LouxTwishi Gulati avatar Twishi Gulatiorcid logoMaria Doyle avatar Maria Doyleorcid logoTristan Reynolds avatar Tristan ReynoldsJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoDelphine Lariviere avatar Delphine LariviereSimon Bray avatar Simon Brayorcid logoErwan Corre avatar Erwan CorreKenji Fujihara avatar Kenji Fujiharaorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoHelena Rasche avatar Helena RascheNathan Dunn avatar Nathan Dunn

Funding

These individuals or organisations provided funding support for the development of this resource