Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoTristan Reynolds avatar Tristan ReynoldsAnton Nekrutenko avatar Anton Nekrutenkoorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoPaul Zierep avatar Paul ZierepEkaterina Polkh avatar Ekaterina Polkhorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoLaura Leroi avatar Laura Leroiorcid logoTeresa Müller avatar Teresa Müllerorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoHelena Rasche avatar Helena Rascheorcid logoSimon Gladman avatar Simon GladmanTwishi Gulati avatar Twishi GulatiTorsten Seemann avatar Torsten Seemannorcid logoSaskia Hiltemann avatar Saskia HiltemannValentin Loux avatar Valentin Louxorcid logoAlexandre Cormier avatar Alexandre CormierHélène Chiapello avatar Hélène ChiapelloWilliam Durand avatar William DurandGuillaume Gautreau avatar Guillaume Gautreauorcid logoMaria Doyle avatar Maria Doyleorcid logoErwan Corre avatar Erwan Correorcid logoNiklas Mayle avatar Niklas Mayleorcid logoDelphine Lariviere avatar Delphine LariviereJasper Ouwerkerk avatar Jasper OuwerkerkNathan Dunn avatar Nathan Dunnorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoAnika Erxleben avatar Anika ErxlebenBazante Sanders avatar Bazante Sandersorcid logoBérénice Batut avatar Bérénice Batutorcid logoMateo Boudet avatar Mateo BoudetKenji Fujihara avatar Kenji Fujiharaorcid logoAnthony Bretaudeau avatar Anthony BretaudeauSimon Bray avatar Simon Brayorcid logoSaim Momin avatar Saim MominNiall Beard avatar Niall Beardorcid logoBjörn Grüning avatar Björn GrüningGildas Le Corguillé avatar Gildas Le CorguilléEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoAnna Syme avatar Anna Symeorcid logoDeepti Varshney avatar Deepti Varshney

Funding

These individuals or organisations provided funding support for the development of this resource