Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoLaura Leroi avatar Laura LeroiGuillaume Gautreau avatar Guillaume Gautreauorcid logoBérénice Batut avatar Bérénice Batutorcid logoAlexandre Cormier avatar Alexandre CormierHélène Chiapello avatar Hélène ChiapelloTwishi Gulati avatar Twishi Gulatiorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoAnna Syme avatar Anna Symeorcid logoPaul Zierep avatar Paul Ziereporcid logoSaim Momin avatar Saim MominEkaterina Polkh avatar Ekaterina Polkhorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoJonathan Kreplak avatar Jonathan KreplakWilliam Durand avatar William DurandGildas Le Corguillé avatar Gildas Le CorguilléTorsten Seemann avatar Torsten Seemannorcid logoMaria Doyle avatar Maria Doyleorcid logoErwan Corre avatar Erwan CorreKenji Fujihara avatar Kenji Fujiharaorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoNiklas Mayle avatar Niklas Mayleorcid logoFelicitas Kindel avatar Felicitas KindelEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilNathan Dunn avatar Nathan Dunnorcid logoDelphine Lariviere avatar Delphine LariviereValentin Loux avatar Valentin LouxBazante Sanders avatar Bazante Sandersorcid logoMateo Boudet avatar Mateo Boudetorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoTeresa Müller avatar Teresa Müllerorcid logoTristan Reynolds avatar Tristan ReynoldsAnton Nekrutenko avatar Anton NekrutenkoSimon Bray avatar Simon Brayorcid logoSimon Gladman avatar Simon Gladmanorcid logoHelena Rasche avatar Helena RascheJasper Ouwerkerk avatar Jasper OuwerkerkNiall Beard avatar Niall Beardorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoBjörn Grüning avatar Björn Grüningorcid logoSaskia Hiltemann avatar Saskia Hiltemann

Funding

These individuals or organisations provided funding support for the development of this resource