Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Hélène Chiapello avatar Hélène Chiapelloorcid logoBjörn Grüning avatar Björn GrüningKenji Fujihara avatar Kenji Fujiharaorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoSimon Gladman avatar Simon Gladmanorcid logoPaul Zierep avatar Paul Ziereporcid logoTristan Reynolds avatar Tristan ReynoldsTorsten Seemann avatar Torsten Seemannorcid logoJonathan Kreplak avatar Jonathan KreplakAnton Nekrutenko avatar Anton Nekrutenkoorcid logoDeepti Varshney avatar Deepti VarshneyWilliam Durand avatar William DurandSimon Bray avatar Simon BrayGuillaume Gautreau avatar Guillaume Gautreauorcid logoNiklas Mayle avatar Niklas Mayleorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoBérénice Batut avatar Bérénice Batutorcid logoMateo Boudet avatar Mateo BoudetJasper Ouwerkerk avatar Jasper OuwerkerkGildas Le Corguillé avatar Gildas Le Corguilléorcid logoAnna Syme avatar Anna SymeEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilBazante Sanders avatar Bazante Sandersorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoTeresa Müller avatar Teresa MüllerTwishi Gulati avatar Twishi Gulatiorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoMiaomiao Zhou avatar Miaomiao ZhouEkaterina Polkh avatar Ekaterina Polkhorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoLaura Leroi avatar Laura Leroiorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoErwan Corre avatar Erwan CorreNiall Beard avatar Niall BeardNathan Dunn avatar Nathan Dunnorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoMaria Doyle avatar Maria DoyleValentin Loux avatar Valentin Louxorcid logoSaim Momin avatar Saim Mominorcid logoHelena Rasche avatar Helena Rascheorcid logoAlexandre Cormier avatar Alexandre Cormier

Funding

These individuals or organisations provided funding support for the development of this resource