Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

Kenji Fujihara avatar Kenji Fujiharaorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoAlexandre Cormier avatar Alexandre CormierGildas Le Corguillé avatar Gildas Le Corguilléorcid logoAnna Syme avatar Anna Symeorcid logoDelphine Lariviere avatar Delphine LariviereJasper Ouwerkerk avatar Jasper OuwerkerkBazante Sanders avatar Bazante SandersTwishi Gulati avatar Twishi GulatiHélène Chiapello avatar Hélène Chiapelloorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoPaul Zierep avatar Paul ZierepValentin Loux avatar Valentin LouxAnton Nekrutenko avatar Anton Nekrutenkoorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoRomane LIBOUBAN avatar Romane LIBOUBANEkaterina Polkh avatar Ekaterina PolkhNiall Beard avatar Niall Beardorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoTeresa Müller avatar Teresa Müllerorcid logoAnthony Bretaudeau avatar Anthony BretaudeauGuillaume Gautreau avatar Guillaume GautreauNathan Dunn avatar Nathan Dunnorcid logoNicola Soranzo avatar Nicola SoranzoWilliam Durand avatar William Durandorcid logoBérénice Batut avatar Bérénice Batutorcid logoMaria Doyle avatar Maria Doyleorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoErwan Corre avatar Erwan Correorcid logoSimon Gladman avatar Simon Gladmanorcid logoBjörn Grüning avatar Björn Grüningorcid logoLaura Leroi avatar Laura LeroiSimon Bray avatar Simon Brayorcid logoHelena Rasche avatar Helena Rascheorcid logoMateo Boudet avatar Mateo BoudetEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoCristóbal Gallardo avatar Cristóbal GallardoTorsten Seemann avatar Torsten Seemannorcid logoSaskia Hiltemann avatar Saskia Hiltemann

Funding

These individuals or organisations provided funding support for the development of this resource