Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Pangenome analysis with Roary
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Community Resources

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoErwan Corre avatar Erwan CorreNiall Beard avatar Niall Beardorcid logoNicola Soranzo avatar Nicola SoranzoTorsten Seemann avatar Torsten Seemannorcid logoMateo Boudet avatar Mateo Boudetorcid logoSimon Gladman avatar Simon Gladmanorcid logoTeresa Müller avatar Teresa MüllerTwishi Gulati avatar Twishi GulatiValentin Loux avatar Valentin Louxorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoMaria Doyle avatar Maria DoyleBazante Sanders avatar Bazante SandersGuillaume Gautreau avatar Guillaume Gautreauorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoBérénice Batut avatar Bérénice BatutJasper Ouwerkerk avatar Jasper OuwerkerkAnton Nekrutenko avatar Anton Nekrutenkoorcid logoLaura Leroi avatar Laura LeroiKenji Fujihara avatar Kenji Fujiharaorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoPaul Zierep avatar Paul ZierepNathan Dunn avatar Nathan Dunnorcid logoSaskia Hiltemann avatar Saskia HiltemannEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoDeepti Varshney avatar Deepti VarshneyHélène Chiapello avatar Hélène Chiapelloorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoNiklas Mayle avatar Niklas Mayleorcid logoSaim Momin avatar Saim MominSimon Bray avatar Simon Brayorcid logoRomane LIBOUBAN avatar Romane LIBOUBANGildas Le Corguillé avatar Gildas Le CorguilléEkaterina Polkh avatar Ekaterina Polkhorcid logoBjörn Grüning avatar Björn Grüningorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoAnna Syme avatar Anna SymeWilliam Durand avatar William Durandorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoHelena Rasche avatar Helena Rasche

Funding

These individuals or organisations provided funding support for the development of this resource